生物信息学技术

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a competitive binding assay for the glycine binding site on the NMDA subtype of glutamate receptors in rat brain

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the preparation of conjugates between nanometer?scale gold particles and synthetic oligonucleotides. Oligonucleotid

Abstract Table of Contents Materials Figures Literature Cited Abstract Despite the advances of non?radioactive methods of protein labeling, radioiodination remains an important tool for studying proteins.

Abstract Table of Contents Materials Figures Literature Cited Abstract A wide variety of xenobiotic carboxylic acids are metabolized to their amino acid conjugates via a pathway that exists primarily in l

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes, in detail, the preparation of 3?aminopropyl?substituted pyrazolopyrimidine analogs of the purines deoxyadenosine

Abstract Table of Contents Figures Literature Cited Abstract This overview unit focuses on the basics of fluorescence and of the FRET phenomenon, and on methods for detecting FRET and data interpretation. FRET is very versatile and there a

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the process and analysis of affinity selecting bacteriophage M13 from libraries displaying combinatorial peptides

Abstract Table of Contents Materials Figures Literature Cited Abstract Liquid chromatography techniques have been successfully coupled with mass spectrometers to provide a robust method for the identifica

Abstract Table of Contents Literature Cited Abstract The analytical ultracentrifuge is a high speed centrifuge with an optical system allowing observation of the concentration of macromolecules as a function of radius and time. In sedimentation velocity experiments,relativ

Abstract Table of Contents Figures Literature Cited Abstract Various types of minor groove binders have been attached to synthetic oligodeoxynucleotides, and the interactions of these conjugates (MB?ODNs) with DNA are reviewed here. MB?ODN

Abstract Table of Contents Materials Figures Literature Cited Abstract Described here are several computational procedures for the analysis of electrostatic interactions in molecular complexes, all based

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a Scintillation Proximity Assay (SPA) for the measurement of ligand binding to melanocortin receptors (MCRs) usin

Abstract Table of Contents Materials Figures Literature Cited Abstract The preparation of two reversible biotinylation phosphoramidites and their application in labeling and affinity purification of synth

Abstract Table of Contents Materials Figures Literature Cited Abstract 5??Iodinated oligodeoxyribonucleotides readily react with 3??phosphorothioated DNA in the presence of a complementary template to yie

Abstract Table of Contents Materials Figures Literature Cited Abstract The conjugation of maleimide reporter groups to 5??diene?modified oligonucleotides via aqueous Diels?Alder cycloaddition is described

Abstract Table of Contents Materials Figures Literature Cited Abstract A facile method for preparation of uridine 2??carbamate derivatives based on reaction of 3?,5??disilyl?protected uridine with 1,1??ca

Abstract Table of Contents Materials Figures Literature Cited Abstract Protein?protein interactions play a critical role in biology. Disrupting a specific protein?protein interaction and studying the resu

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes procedures for performing competition binding assays with neurotensin receptor subtypes 1 and 2 (NTS1 and NTS2).

Abstract Table of Contents Figures Literature Cited Abstract In recent years, optical evanescent wave biosensors have been used to characterize protein?protein interactions, including determination of equilibrium binding constants and bimo

Abstract Table of Contents Materials Figures Literature Cited Abstract The aim of this unit is to provide a method for the identification of new protein?protein interactions. Pull?down experiments with GS