生物信息学技术

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a number of methods for modifying cysteine residues of proteins and peptides by reduction and alkylation procedur

Abstract Table of Contents Materials Figures Literature Cited Abstract One of the most versatile and accessible types of isolated tissue used in pharmacology is the isolated blood vessel. Arteries and vei

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes assays for measuring the binding of radioligands to two major types of receptors for 5?hydroxytryptamine (5?HT or

Abstract Table of Contents Materials Figures Literature Cited Abstract The N?methyl?D?aspartate subtype of glutamate receptor is unusual in that it requires two endogenous agonists for activation. Thus, in

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit provides an introduction to the binding of specific ligands to K+ channels that are expressed in a number of different prepar

Abstract Table of Contents Materials Figures Literature Cited Abstract The mammalian tachykinin peptides are a family of neuropeptides characterized by a common C?terminal amino acid sequence of the form

Abstract Table of Contents Materials Literature Cited Abstract The ubiquitous distribution of EAA receptors results from the fact that EAA?mediated neurotransmission is the most prevalent type of fast, chemically?mediated communication in

Abstract Table of Contents Materials Figures Literature Cited Abstract Corticotropin?releasing factor (CRF) and its receptors play a major role in regulating an organism's response to physical, emotional

Abstract Table of Contents Materials Figures Literature Cited Abstract Cholecystokinin (CCK) and gastrin are structurally related peptide hormones synthesized and secreted by endocrine cells in the gastri

Abstract Table of Contents Materials Figures Literature Cited Abstract Neuropeptide Y (NPY), peptide YY (PYY), and pancreatic polypeptide (PP) constitute a trio of structurally related peptides that elici

Abstract Table of Contents Materials Figures Literature Cited Abstract Angiotensin II (Ang II) is the primary mediator of the renin angiotensin system and acts primarily as a circulating hormone, but may

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the preparation and applications of porphyrinic sensors for quantitative measurement of nitric oxide (NO) in sing

Abstract Table of Contents Materials Figures Literature Cited Abstract Nitric oxide (NO) is a key biosignaling molecule produced in both peripheral tissues and the central nervous system by a family of en

Abstract Table of Contents Materials Literature Cited Abstract cAMP acts as an intracellular mediator of hormone action and the importance of accurate quantitative determination of cAMP levels in cells and tissues is widely recognized. The

Abstract Table of Contents Materials Figures Literature Cited Abstract Ca2+, Na+, K+, and Mg2+ have a central role in neuronal excitability. The concentration of these cations in the cytoplasm of neurons

Abstract Table of Contents Materials Figures Literature Cited Abstract In this unit, protocols are described for biochemical and optical techniques that have been used by investigators to measure ligand?g

Abstract Table of Contents Materials Figures Literature Cited Abstract The availability of clonal cell lines for norepinephrine, dopamine, and serotonin transporters allows the characterization of drug in

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the use of a Cytosensor microphysiometer for functional characterization of an agonist and antagonist to a G prot

Abstract Table of Contents Materials Figures Literature Cited Abstract The use of filtration assays is often unsuitable for radioligands with rapid dissociation rates. Rapid dissociation may result in the

Abstract Table of Contents Materials Figures Literature Cited Abstract The protocols in this unit describe methods for measuring radioligand binding to sites on the GABA?A receptor complex. This system wa