生物信息学技术

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes two basic protocols for the detection of the proliferation of neural stem cells (NSC). The first one addresses ce

Abstract Table of Contents Materials Figures Literature Cited Abstract Research models that replicate the diverse genetic and molecular landscape of breast cancer are critical for developing the next?gene

Abstract Table of Contents Materials Figures Literature Cited Abstract Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation resulting from inflammation?driven pa

Abstract Table of Contents Materials Figures Literature Cited Abstract Human asthma is defined as a chronic inflammatory disease of the airways. Animal models are required to study asthma pathophysiology

Abstract Table of Contents Figures Literature Cited Abstract Type 1 diabetes (T1D) is characterized by a partial or total insufficiency of insulin. The premiere animal model of autoimmune T cell?mediated T1D is the NOD mouse. A dominant ne

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes a streamlined two?step protocol for the isolation of adult murine cardiomyocytes with subsequent Chromatin Immuno

Abstract Table of Contents Materials Figures Literature Cited Abstract At its most ambitious, untargeted metabolomics aims to characterize and quantify all of the metabolites in a given system. Metabolite

Abstract Table of Contents Materials Figures Literature Cited Abstract Major depression is a complex psychiatric disorder characterized by affective, cognitive, and physiological impairments that lead to

Abstract Table of Contents Materials Figures Literature Cited Abstract Besides hematological analyses, many other parameters, including clinical chemistry and endocrinological values, can be determined fr

Abstract Table of Contents Materials Figures Literature Cited Abstract Many genetic and environmental perturbations lead to measurable changes in bone morphology, matrix composition, and matrix organizati

Abstract Table of Contents Materials Figures Literature Cited Abstract Glioblastoma multiforme (GBM) is one of the most common and most malignant of the brain tumors. Gliomas can be classified into four d

Abstract Table of Contents Materials Figures Literature Cited Abstract Animal behavioral studies have commonly regarded the entire group of animals subjected to the study conditions as homogeneous, disreg

Abstract Table of Contents Materials Figures Literature Cited Abstract While fear and anxiety can grow over time in anxiety disorders, most efforts to model this phenomenon with fear conditioning in roden

Abstract Table of Contents Materials Figures Literature Cited Abstract Customized DNA?binding domains made using transcription activator?like effector (TALE) repeats are rapidly growing in importance as w

Abstract Table of Contents Materials Figures Literature Cited Abstract ATP?binding cassette (ABC) proteins, including the breast cancer resistance protein (BCRP) and multidrug resistance proteins (MDRs),

Abstract Table of Contents Materials Figures Literature Cited Abstract Glutathionylation is a posttranslational modification that results in the formation of a mixed disulfide between glutathione and the

Abstract Table of Contents Materials Figures Literature Cited Abstract High?throughput short?hairpin RNA (shRNA) lentivirus screening is a powerful tool for identifying multiple functional regulators in e

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNP

Abstract Table of Contents Materials Figures Literature Cited Abstract This unit describes our current knowledge regarding the isolation human bone marrow?derived progenitor cells for the paracrine stimul

Abstract Table of Contents Materials Figures Literature Cited Abstract Airway sensory nerves play an important defensive role in the lungs, being central in mediating protective responses like cough and b