植物学技术

During development of multicellular organisms, cells become differentiated by modulating different programs of gene expression. Cells have their own epigenetic signature which reflects genotype, developmental history, and environmental influences, and it is ultimate ...

A protocol for the induction of a cell suspension culture of Argemone mexicana is described. This suspension has been kept for over 3 years producing sanguinarine, a benzophenanthridine-type alkaloid. Sanguinarine levels can be increased by exposing these cultures to yeast or fungal el ...

In vitro roots cultures of Typha latifolia and Scirpus americanus aquatic plants have the capacity to remove Pb (II), Mn (II), and Cr (III) from the culture medium. Both species remove Cr and Pb by an absorption process, while Mn is mainly adsorbed to the root surface. This chapter describes a protocol for the ...

Plant cell, tissue, and organ culture (PTC) techniques were developed and established as an experimental necessity for solving important fundamental questions in plant biology, but they currently represent very useful biotechnological tools for a series of important applicati ...

The ability of plants to remove pollutants from the environment is currently used in a simple and low-cost cleaning technology known as phytoremediation. Unfortunately, little is known about the metabolic pathways involved in the transformation of xenobiotic compounds and the abil ...

In breeding of ornamental plants, interspecific hybridization and polyploidization have successfully been used to produce novel cultivars with blended traits of both parents and to introgress useful traits of one species to another. Embryo rescue techniques and molecular cytog ...

Chili pepper (Capsicum spp.) is a very important horticultural crop around the world and is especially important for Mexicans because of its impact in the culture and the cuisine. Biotechnological tools such as tissue culture techniques and specifically anther culture may be applied suc ...

Cryopreservation is the storage of viable bioresources at ultra-low temperatures in liquid nitrogen (LN). This chapter provides an overview of those protocols most commonly used to cryopreserve in vitro derived shoot tips and meristems; they are described generically, as sequenti ...

Arabidopsis thaliana is an excellent experimental system to study various aspects of plant biology. An efficient regeneration protocol of the plant is important to produce in vitro fertile plants, as well as a fundamental step to study the cellular, physiological, and molecular mechani ...

Plant regeneration capacity is maintained through the life of a plant by the stem cell niche present in the meristems. Stem cells are capable of differentiating into any plant organ, allowing propagation of new plants by different techniques. Among them, somatic embryogenesis is a widely used ...

The in vivo haploid induction approach offers several advantages compared to the in vitro induction approach and recurrent self-pollination. It is currently the method of choice for inbred line development in many commercial maize breeding programs. Here, we describe the in vivo approa ...

We describe a simple and inexpensive plant micropropagation system for giant reed (Arundo donax L.) that uses axillary buds from the lateral stems of elite plants selected from field- or nursery-grown plants. The buds, attached to the stems are cultured in stationary liquid MS culture medium, s ...

Banana (Musa spp. AAA) is propagated vegetatively and can be rapidly and efficiently propagated by micropropagation. Conventional micropropagation techniques, however, may be too costly for commercial purposes. Our laboratory has found that depending on the combination of cult ...

The choice of a method to culture red cedar tissues depends on the final objectives pursued. If homogeneous clonal material is required for experimental purposes, the easiest way is to generate the lines through adventitious shoot induction from seedlings germinated from seeds. If the obj ...

Functional redundancy widely exists in genes encoding receptors and signaling components of plant hormones, particularly the stress hormone abscisic acid (ABA). The redundancy hinders the use of conventional genetic approach to dissecting molecular mechanisms for ABA signal ...

Insertional mutagenesis is one of the most effective approaches to determine the function of plant genes. However, due to genetic redundancy, loss-of-function mutations often fail to reveal the function of a member of gene families. Activation tagging is a powerful gain-of-function app ...

The growth-promoting hormones, the brassinosteroids (BRs), are perceived at the plant cell surface by receptor kinases that transduce the signal to the nucleus by an intracellular cascade of phosphorylation-mediated protein–protein interactions. BR signaling is also regula ...

Environmental stresses often trigger rapid generation of reactive oxygen species (ROS) in plants. Excessive amount of ROS can cause damage to plant cells and thus need to be counteracted by cellular antioxidant systems. On the other hand, ROS also serve as signaling molecules that modulate v ...

The plasma membrane (PM) controls cell’s exchange of both material and information with the outside environment, and PM-associated proteins play key roles in cellular regulation. Numerous cell surface receptors allow cells to perceive and respond to various signals from neighbor ce ...

Posttranslational modifications of proteins, especially phosphorylation and dephosphorylation, play an important role in signal transduction and cellular regulation in plants. Both 2-DE gel-based and non-gel-based proteomic technologies can monitor the changes in pho ...