植物学技术

Although proteomists working with gel-free methods are considering the gels as coming from the past, proteomics based on gels has still a lot of opportunities to offer and acquisition of images on which thousands of spots may be resolved is still largely performed. Nowadays, two-dimensional ...

The use of analytical biochemical techniques with different separation properties allows us to better understand the proteome. To demonstrate this we have used two different methodologies to analyze embryos from a Tunisian cultivar of durum wheat (Triticum durum Desf.), variety Oum R ...

Mass spectrometry (MS) is an analytical technique that measures the mass-to-charge ratio of electrically charged gas-phase particles. All mass spectrometers combine ion formation, mass analysis, and ion detection. Although mass analyzers can be regarded as sophisticated devic ...

Two-dimensional gel electrophoresis remains the most widely used technique for protein separation in plant proteomics experiments. Despite the continuous technical advances and improvements in current 2-DE protocols, an adequate and correct experimental design and stati ...

Two-dimensional electrophoresis has nurtured the birth of proteomics. It is however no longer the exclusive setup used in proteomics, with the development of shotgun proteomics techniques that appear more fancy and fashionable nowadays. Nevertheless, 2D gel-based proteomics st ...

Proteomic approaches are useful for the identification of functional proteins. These have been enhanced not only by the development of proteomic techniques but also in concert with genome sequencing. In this chapter, 30 databases and Web sites relating to plant proteomics are reviewed a ...

Genome sequencing and systems biology are revolutionizing life sciences. Proteomics emerged as a fundamental technique of this novel research area as it is the basis for gene function analysis and modeling of dynamic protein networks. Here a complete proteomics platform suited for fun ...

In this first, introductory chapter, it is intended to summarize from a methodological point of view the state of the art in plant proteomics, focusing on mass spectrometry-based strategies. Thus, this chapter is mainly directed at beginners or at those trying to get into the field, rather than at th ...

The Mutator system has proved to be an invaluable tool for elucidating gene function via insertional mutagenesis. Its high copy number, high transposition frequency, relative lack of insertion specificity, and ease of use has made it the preferred method for gene tagging in maize. Recent adv ...

Localizing Ac insertions is a fundamental task in studying Ac-induced mutation and chromosomal rearrangements involving Ac elements. Researchers may sometimes be faced with the situation in which the sequence flanking one side of an Ac/Ds element is known, but the other flank is unknown. Or, a ...

We describe here protocols for isolating genes in maize using Dissociation (Ds) transposons marked with a green fluorescent protein (GFP) transgene. The introduced marker enables the phenotypic scoring of the nonautonomous element and the anchoring of unique primers on the element to ...

To mutagenize rice genomes, a two-element system is utilized. This system comprises an immobile Ac element driven by the CaMV 35S promoter, and a gene trap Ds carrying a partial intron with alternative splice acceptors fused to the GUS coding region. Rapid, large-scale generation of a Ds transpos ...

Maize Activator (Ac) is one of the prototype transposable elements of the hAT transposon superfamily, members of which were identified in plants, fungi, and animals. The autonomous Ac and nonautonomous Dissociation (Ds) elements are mobilized by the single transposase protein encoded ...

Marker genes have played a critical role in the discovery of plant transposable elements, our understanding of transposon biology, and the utility of transposable elements as tools in functional genomics. Marker traits in model plants have been useful to detect transposable elements a ...

Variable phenotypes are common in nature and in laboratory materials. Guidelines and illustrations are presented to help distinguish developmental, environmental, disease, and somatic recombination-generated variation from the phenotypes caused by transposable ele ...

Genetic mosaics, or chimeras, are individual organisms composed of cells or tissues of two or more distinct genotypes. They are experimentally useful for addressing several key biological questions. These include fate mapping through analysis of marked clonal lineages, analyzing ...

Grass genomes harbor a diverse and complex content of repeated sequences. Most of these repeats occur as abundant transposable elements (TEs), which present unique challenges to sequence, assemble, and annotate genomes. Multiple copies of Long Terminal Repeat (LTR) retrotranspos ...

The initial identification of transposable elements (TEs) was attributed to the activity of DNA transposable elements, which are prevalent in plants. Unlike RNA elements, which accumulate in the gene-poor heterochromatic regions, most DNA elements are located in the gene rich regions ...

The availability of a large amount of genomic sequences has provided unique opportunities for understanding the composition and dynamics of transposable elements (TEs) in plants. As the cost of sequencing declines, the genomic sequences of most crop plants will be available within the n ...

Research on transposable elements began nearly 100 years ago with classical genetic experiments. Remarkably, many of the activities of transposable elements, such as the ability to transpose, to induce chromosome rearrangements, to undergo cycles of activity and inactivity, and to a ...