【目的】通过实验学习测定药物LD50的方法、步骤及计算过程,观察受试药品一次给予动物后所产生的急性毒性反应和死亡情况。 【原理】药物给药剂量与动物死亡率间呈正态分布,以对数剂量为横坐标、死亡率为纵坐标作图,可得到一对称S型曲线,其两端较平坦,中间较陡,说明两端处剂量稍有变化时死亡率的改变不易表现出来,在50%死亡率处斜率最大,该处剂量稍有变动时,其死亡率变动最明显,即最灵敏,在技术上也最容易测得准 ...
关键词:尿液采集 操作规程 目的:采集各种实验动物的尿液以用于实验 主体内容: 常用的采集方法较多,一般在实验前需给动物灌服一定量的水。 (一)代谢笼法:此法较常用,适用于大、小鼠。将动物放在特制的笼内。动物排便时,可以通过笼子底部的大小便分离漏斗将尿液与粪便分开,达到采集尿液的目的。 由于大、小鼠尿量较少,操作中的损失和蒸发,各鼠膀胱排空不一致等原因,都可造成较大的误差,因此一般需收集5小时以上 ...
关键词:生物样品的采集 实验动物 解剖 脏器系数测定 目的:在药物毒理学研究中,常常需要收集实验动物的血液、尿液或其它体液进行常规检查或生化分析,因此,正确采集实验动物的生物材料是药物毒理学最基本和最重要的操作技术。对实验动物进行大体解剖检查是药物毒理实验的常规观察项目,简便易行并能提供重要资料。测定动物死后器官湿重和含水量是常用的指标之一,可以从大体标本大概地估计内脏器官病变的程度,特别适用于某 ...
关键词:大鼠 新生鼠 灌注取脑 后固定 目的: 完整取出鼠脑及后期固定 操作步骤 流程:1)麻醉 2)开胸 3)心脏解剖 4)生理盐水冲水 5)4%多基甲醛固定 6)取脑 7)保存或切片. 1 多聚甲醛的配置: 一般方法为:4%多聚甲醛PBS缓冲液配法:称取40g PFA溶于装有800ml PBS的玻璃容器(烧杯或烧瓶)中,持续加热磁力搅拌至60~65℃,使成乳白色悬液。用1.0mol/L的NaO ...
目的:制备小鼠角叉菜胶性足肿模型,筛选抗炎药的抗炎作用 关键词:小鼠 角叉菜胶 足肿 炎症 抗炎 原理:角叉菜胶为常规致炎剂小鼠足跖皮下注射能引起炎症渗出使致炎侧重量增加称取2侧足重的差别即可考察炎症的程度和抗炎药的作用. 主体内容: 一、模型制备 体重18~22 g的昆明种小鼠,♀♂不拘,随机分组,左后肢分别im 受试药、地塞米松 2 mg/kg或生理盐水(或溶媒),于给药后30 min时在每鼠 ...
关键词: 小鼠明暗穿箱实验 目的:本操作规程是关于利用小鼠明暗穿箱实验对受试物的抗焦虑活性进行药理学评价的具体操作规程。 背景知识:无 原理(选填项目):动物置于明箱中央,背朝隔板。暗箱红灯不通电,明箱白炽灯照明。造成动物焦虑。 主体内容(操作步骤): 实验的准备 实验的器材、仪器、药品 仪器:分析天平,摄像仪,录像机,监视器,小鼠明暗箱(为一个上面敞开的箱子(45cm×27cm&ti ...
名称:公用动物实验室标准操作及管理规程(SOP) 关键词:实验动物 实验室 操作管理规则 目的:严格控制动物实验环境不受病源微生物的危害;保护动物的福利;维持环境的生物洁净度;确保动物使用与实验环境的一致性。 背景知识(选填项目): 原理(选填项目): 主体内容: (1)实验人员和本室工作人员进入开放系统动物室,应着工作服,并保持动物饲养室和公用动物实验室的整洁、安静。无关人员未经许可,不得进入。 ...
TITLE: Document Controls SCOPE: All Divisional Personnel RESPONSIBILITY: Program Administrative Personnel PURPOSE: To Outline the Proper Procedures to Control all Policy Procedureand Recording Documen ...
TITLE: Facilities for Aseptic Surgery SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Identify the Available Surgica ...
TITLE: Pest Control SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline the Program of Pest Control Within An ...
TITLE: Animal Quarantine Stabilization and Separation SCOPE: All Animal Program Personnel RESPONSIBILITY: Veterinary Staff Facility Manager Surgery Core Manager All Animal Personnel PURPOSE: To Out ...
TITLE: Animal Health and Environmental Surveillance SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Daily Review Ani ...
TITLE: Transportation Relocation or Reassignment of Animals SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager All Animal Program Personnel PURPOSE: To Outline the Proper Procedur ...
TITLE: Test and Control Articles SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel Study Sponsor PURPOSE: To Outline the Methods ...
TITLE: Clinical Laboratory Sample Testing SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline the Procedures ...
TITLE: Handling Storage and Retrieval of Records and Data SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline ...
TITLE: Reagent Labeling SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Ensure Uniformity of Labeling of All Regents ...
TITLE: Animal Medical Records for Nonrodent Mammals SCOPE: All Animal Program Personnel RESPONSIBILITY: Clinical Veterinarians Facility Manager Veterinary Care Managerall Research and Animal Personnel ...
TITLE: Employee Orientation and Training SCOPE: All Animal Program Personnel RESPONSIBILITY: Facility Manager Surgical Core Manager All Animal Program Personnel PURPOSE: To Outline the Proper Proce ...
TITLE: Controlled Substances SCOPE: Research and Animal Program Personnel RESPONSIBILITY: Program Coordinator Veterinary Care Manager Facility Managers and All Animal Program Personnel URPOSE: To O ...