实验动物技术

Background:Zebrafishor the teleost fish Danio reriois a rapidly developing organism that is a popular species for studying vertebrate development. Cleavage in the Zebrafish only occurs in the blastodi ...

Gastrulation has been celled "the most important time of your life" (Lewis Wolpert); without it you would be flat like a pancake! Vertebrate gastrulation is the process by which the simple structure o ...

IntroductionIn early stages of development in amphibian embryos very few cells' fates are rigidly determined. However previous experiments show that cells in one area of the developing embryo are dete ...

EMBRYO CELL AND TISSUE CULTURE TECHNIQUESRearing solution: 10% Modified Steinberg's Solution. Operating solution:Full strength HBSt solution. Microsurgery Preparation 1)Place two embryos in the 60 mm ...

Objective:The overall objective of this laboratory experiment is to examine specification in the axolotl through transplantation of the gill organ field.Introduction:The axolotl (Ambystoma mexicanum) ...

From the various microsurgical procedures of Spemann and others several developmental principles have emerged about amphibian embryos. One of the most important discoveries was the understanding that ...

ObjectiveIn this experiment a region of the neural crest will be transplanted from one stage-fourteen embryo to another (Figure 1). To facilitate the visualization of the grafts and observe the cell f ...

IntroductionThere are two modes of development common to most species in the animal kingdom.Virtually all embryos undergo both mosaic and regulative development at some point during their growth. Regu ...

ObjectiveThis experiment will seek to demonstrate regulative development in Ambystoma mexicanum embryos. Specifically we will split the morphogenetic field that is responsible for heart formation usin ...

Cyclopamine and jervine teratogens derived from the skunk cabbage Veratrum californicum are known to induce holoprosencephaly in chick embryos when introduced prior to gastrulation. Holoprosencephaly ...

ObjectiveThis experiment attempted to determine the effects of the teratogen cyclopamine on the Shh signaling pathway in axolotl embryos. Groups of axolotl embryos that had undergone gastrulation were ...

PURPOSE: The objective of this experiment is to study the effect of lithium chloride treatment on early blastula xenopus embryos.INTRODUCTION: When the developing embryos of many organisms such as the ...

The development of chick embryos has been studied since Aristotle. It is one of the most intensely studied organisms. One reason for this is that there are great similarities between avian and mammali ...

This experiment allows you to observe the development of a chick embryo outside its shell. Cultured chicks are also more accessible for manipulation. Compare the extent of bone mineralization of contr ...

In order to repeat a published experiment or have someone else repeat yours it is important to use the same materials. For developmental studies that means knowing the precise point in development tha ...

ABSTRACTFibroblast growth factor (FGF) signaling plays a signifigant role in embryonic development. Particularyl FGFs are implicated in mediating epithelial-mesenchymal interactions by means of regula ...

ObjectiveThis experiment explores the ability of the chick chorioallantoic membrane (CAM) to support an excised limb bud from a donor embryo. The chick system will allow observation of general cartila ...

Abstract Choriallantoic membrane (CAM) limb grafting functions as a method to isolate the inductive events of limb formation. Experimenters have the ability to isolate and culture tissues outs ...

The chicken is a classic organism used to illustrate the principles of basic embryology. One of the developmental systems which has been examined in great detail is the circulatory system. In the deve ...

Objective:The purpose of this experiment was to explore the effects of the growth factors bFGF and VEGF on blood vessel formation within the chorioallantoic membrane (CAM) of chick embryos. Background ...