实验动物技术

大鼠4-羟基壬烯醛（4-HNE）酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定大鼠血清，血浆组织及相关液体样本中4-羟基壬烯醛（4-HNE）的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠4-羟基壬烯醛（4-HNE）水平。用纯化的大鼠4-羟基壬烯醛（4-HNE)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入4-羟基壬烯醛（4 ...

大鼠5α-还原酶Ⅱ酶联免疫分析试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定大鼠血清，血浆，细胞上清及相关液体样本中5α-还原酶Ⅱ的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠5α-还原酶Ⅱ水平。用纯化的大鼠5α-还原酶Ⅱ抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入5α-还原酶Ⅱ，再与HRP标记的5α-还原酶Ⅱ抗体结合，形成抗体-抗原-酶标抗体复 ...

大鼠Ⅲ型胶原（ColⅢ）酶联免疫分析试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定大鼠血清，细胞上清及相关液体样本中Ⅲ型胶原（ColⅢ）的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠Ⅲ型胶原（ColⅢ）水平。用纯化的大鼠Ⅲ型胶原（ColⅢ）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Ⅲ型胶原（ColⅢ），再与HRP标记的Ⅲ型胶原（Col ...

大鼠Ⅲ型前胶原（PCⅢ）酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中Ⅲ型前胶原（PCⅢ）的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠Ⅲ型前胶原（PCⅢ）水平。用纯化的大鼠Ⅲ型前胶原（PCⅢ）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入Ⅲ型前胶原（PCⅢ），再与HRP标记的Ⅲ型前胶原（PCⅢ ...

大鼠APJ酶联免疫分析（ELISA）试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本中APJ的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠APJ水平。用纯化的大鼠APJ抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入APJ，再与HRP标记的APJ抗体结合，形成抗体-抗原-酶标抗体复合物，经过彻底洗涤后加底物TMB显色。TMB在 ...

大鼠肿瘤坏死因子α（TNF-α）酶联免疫分析(ELISA)试剂盒使用说明书本试剂仅供研究使用目的：本试剂盒用于测定大鼠血清，组织及相关液体样本中肿瘤坏死因子α（TNF-α）的含量。实验原理：本试剂盒应用双抗体夹心法测定标本中大鼠肿瘤坏死因子α（TNF-α）水平。用纯化的大鼠肿瘤坏死因子α（TNF-α）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入肿瘤坏死因子α（TNF- ...

一、常规基因敲除鼠（Conventional Knockout）常规基因敲除是通过基因打靶，把需要敲除的基因的几个重要的外显子或者功能区域用Neo Cassette 替换掉。这样的小鼠其全身所有的组织和细胞中都不表达该基因产物。此类基因敲除鼠一般用于研究某个基因在对小鼠全身生理病理的影响，而且这个基因没有胚胎致死性。二、条件性基因敲除小鼠（Conditional Knockout）条件性基因敲除小 ...

1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fasten ...

ABSTRACT Knowledge of the geneological relationships of cells during development can allow one to gain insight into when and where developmental decisions are being made. Geneological relationships ca ...

Procedures for In Vitro Production of Bovine Embryos The procedures for in vitro production in vitro fertilization (IVF) and in vitro culture (IVC) of embryos described here are based on procedures d ...

8 eggs per day day 7- day 13 cut CAM wash in precooled PBS in 10 ml WASH 1 (PBS 5 mM EDTA COMPLETE) on ice cut in pieces in petri dish on ice centrifuge 4 min at 1000 rpm (table top centifuge Falcon 1 ...

1. Sacrifice impregnated mouse.2. Dissect out the uterus of the mouse. Pulling up on the uterus with one set of forcepsuse another to tear the mesometrium away from the uterus.3. Remove the embryos fr ...

1. The basic unit is constructed from a media filtration unit such as Corning’s 115 ml filter system (catalog numbers 430944-430947). 2. Tygon tubing (3.2 mm i.d. x 6.4 mm o.d.) with a stopcock fasten ...

MEMFA Fix10xMEMFA Salts1 part 10x MEMFA salts1 M MOPS1 part 37% formaldehyde20mM EGTA8 parts water10mM MgSO410x salts can be autoclaved and stored. Turns yellow after autoclaving. ...

Ovulation is induced in X. tropicalis by injection of human chorionic gonadotropin (hCG). X. tropicalis requires a much smaller dose than does X. laevis. We use basically the same protocol as that des ...

When we first started using X. tropicalis in vitro fertilization had an extremely poor efficiency. However with the careful selection of a mature male and the use of L15/CS (L15 Leibovitch's Media sup ...

In order to get the best in vitro fertilization two criteria appear to be critical: first selection of a mature male and second use of L15/CS (L-15 Medium supplemented to 10% calf serum).Select a mal ...

For natural matings single pairs of boosted males and females are placed together. We usually keep them on a darkened shelf away from disturbances but where they can be easily monitored. Three to four ...

Four hours after boosting with hCG the females are ready to begin laying eggs. The best test is to see if there are eggs in the holding buckets. Also the cloaca gets red and swollen like in X. laevis. ...

Spawning can be induced in adult sea urchinsby injecting 1 ml of a0.5M KCl solution into several sites in the soft membrane around the mouth. Within minutes the gametes should appear: the sperm are of ...