实验动物技术

In order to get the best in vitro fertilization two criteria appear to be critical: first selection of a mature male and second use of L15/CS (L-15 Medium supplemented to 10% calf serum).Select a mal ...

For natural matings single pairs of boosted males and females are placed together. We usually keep them on a darkened shelf away from disturbances but where they can be easily monitored. Three to four ...

Spawning can be induced in adult sea urchinsby injecting 1 ml of a0.5M KCl solution into several sites in the soft membrane around the mouth. Within minutes the gametes should appear: the sperm are of ...

Gametes are collected as described. To remove some of their jelly coatwash eggs several times in ASW by allowing them to settle and gently pouring off excess liquid. Transfer enough eggs to barely cov ...

Histochemical staining of sea urchin embryos for alkaline phosphatase (AP) enzyme activity1. Obtain embryo samples tube of AP substrate buffer and tube of phosphate buffered saline (PBS) for each grou ...

The following is written to help non-marine biologists use sea urchins occasionally in teaching; disregard if you have running sea water tanks in your lab!Gravid animals can be obtained virtually year ...

Invertebrate embryos develop at different rates depending on the temperature of incubation. Descriptive stages based on the number of cells and their arrangement in the embryo are used to enable resea ...

Objective: to test the effects of inhibiting the fast and slow blocks to polyspermy on sea urchin development. BackgroundSea urchin eggs release components of the egg jelly that attract sperm and indu ...

Purpose This experiment will study effects of lithium chloride on sea urchin development forcusing on archenteron formation. IntroductionGastrulation is extensive cell rearrangement where cells underg ...

The Effects of NiCl2on Spicule FormationJessica Ann Billet Franklin and Marshall Class of 2000Background and ObjectiveSea urchins exhibit radial holoblastic cleavage eventually forming a blastula. Sho ...

ObjectiveThe purpose of this experiment is to determine the effects of calcium-free seawater on the development of sea urchin embryos. Embryos were placed in calcium-free sea after fertilization and a ...

Objective: to separate the Blastomere at different points in development and observe the growth of the resulting embryos. Procedure: We extractrf the gametes from the sea urchins fertilized the sea ur ...

Objective: This experiment aims to discover if temperature has an effect on the rates of sea urchin embryonic development. By altering the external environment the experiment will determine if tempera ...

1. Dechorionate 26 hr embryos (pharyngula stage) carefully with two fine forceps.Transfer to fixative (1% formaldehyde in PBS). Fix for 1 hour rocking at 4oC.2. Wash with 5 ml 0.1% BSA in PBS for 10 m ...

In order to repeat a published experiment or have someone else repeat yours it is important to use the same materials. For developmental studies that means knowing the precise point in development tha ...

AbstractPrevious studies by Stachel and colleagues indicate that lithium chloride induction of post-midblastular Brachydanio rerio embryos results in deficiencies in normal anterior-posterior developm ...

Introduction:The early stages of zebrafish Danio rerio development are characterized by meroblastic dicoidal cleavage. Initially cell division only occurs in the blastodisc which is a thin region on t ...

Objective The objective of the experiment is to determine the effects of ethanol exposure on the embryonic development of zebrafish through observation of physical defomities.IntroductionEmbryonic dev ...

BackgroundZebrafish Brachydanio rerio have a distinct embryonic development. The embryo goes through a process called epiboly. Epiboly as defined in the sixth edition of Developmental Biology(Gilbert ...

Background:Zebrafishor the teleost fish Danio reriois a rapidly developing organism that is a popular species for studying vertebrate development. Cleavage in the Zebrafish only occurs in the blastodi ...