微生物学技术

Lymph nodes are the primary sites of T-cell stimulation by dendritic cells (DC). After contact with antigens, DCs migrate to draining lymph nodes from the skin and other tissues (1-3). Investigation of the morphology and function of lymph node DCs may provide important information about the role ...

The method described in this chapter for the isolation of mouse thymic dendritic cells (DC) is an optimization of our previously published methods (1,2) and involves the following major steps:

Dendritic cells (DC) are rare cells in peripheral tissues, and their isolation from tissues is fraught with problems. Thus, the proportion of DC within a tissue that is extracted is unknown, isolation procedures may select for subpopulations, and the isolation procedure itself may affect th ...

Langerhans cells are the epidermal variant of the dendritic cell system (1-5). They were-unknowingly, though-the first dendritic cell to be described: In 1868 Paul Langerhans published his observations of a dendritically shaped cell in the human epidermis (6). Until the early 1990s (i.e., the ...

Dendritic cells (DC) are a trace cell population found in most tissues that display potent antigen acquisition and presentation capabilities and are unique among antigen-presenting-cell types in their ability to activate unprimed T cells (1). DC are particularly prominent at the muco ...

This chapter describes techniques for the isolation of dendritic cell-enriched single-cell suspensions from the lamina propria (LP) of both small and large intestine and from Peyer’s patches (PP). This technique can also be used to obtain intestinal macrophage-enriched populatio ...

Dendritic cells (DC) are highly specialized antigen-presenting cells (APC) derived from precursors within the bone marrow (BM). They are distributed ubiquitously throughout the body, and are few in number (1). They are classified as lymphoid-related or myeloid DC depending on their dev ...

It is well established that dendritic cells (DC) exhibit different phenotypes and functions as they progress down the developmental pathway toward interdigitating DC that stimulate T cells in the secondary lymphoid tissue (1). In the blood, two populations of DC can be identified based on the ...

Tonsillectomy remains a frequently performed operation in developed countries ensuring that tonsils are the most readily available source of human lymphoid tissue and an easily accessible source of dendritic cells (DC). Tonsil lymphoid tissue also provides a source of the different ...

In the human skin, various types of antigen-presenting cells (APC) are present. In the epidermis, they are identified ultrastructurally as Langerhans cells (LC) by the presence of Birbeck granules. LC are considered to belong to the family of dendritic cells (DC) that are important for the initi ...

The skin acts as a mechanical, physicochemical, and immunological control and defense system. The efficient operation of the skin immune system involves cytokine production and adhesion molecule expression by both infiltrating and resident cutaneous cells, and thus depends upon a s ...

Cells with the morphology of veiled cells were first described in the human intestinal lamina propria in tissue obtained from patients suffering from inflammatory bowel diseases (1,2). These cells were found in greater numbers in inflamed bowel than in normal controls. The isolation of den ...

In the lung several cell types are capable of presenting antigen to T cells. The dendritic cells (DC) are the most potent antigen-presenting cell. DC form a rare cell population in the lung and early studies were hampered because scarce cell populations are seldom easy to isolate. Besides, this cell is p ...

In rheumatoid arthitis patients, three compartments need to be considered: peripheral blood (PB), synovial fluid (SF), and synovial tissue (ST). Dendritic cells (DC) characterized from each compartment have different properties. The methods given are based on cell sorting for isolat ...

Dendritic cells (DC) form a complex network of cells that are distributed throughout the body and whose primary function is the stimulation of antigen-specific immune responses (1). DC are derived from CD34+ progenitor cells located in the bone marrow (2) and undergo a complex process of matura ...

The earliest lymphoid precursor in the adult mouse thymus, the “low CD4 precursor,” was found to be able to produce T cells, B cells, NK cells, and dendritic cells (DC) upon adoptive transfer (1-3). This precursor population represents only 0.03%-0.05% of total thymocytes and expresses low levels of CD4 ...

It has long been known that tissue dendritic cells (DC) are bone-marrow-derived (1,2). However, early attempts to generate DC from the bone marrow gave only low numbers, presumably because maturation and survival signals required in vivo could not be provided in vitro (3,4). The availability of r ...

Dendritic cells (DC) are the most potent antigen-presenting cells (APC) of the immune system. Derived from stem cells in the bone marrow, DC migrate to the tissues where they become “sentinels” of the immune system. DC possess the unique ability to initiate primary T-cell responses and can also stim ...

Dendritic cells (DC) are now recognized as major players in the control of immune responses (1), since they direct both the quality and the extent of the adaptative response. Thus, DC represent a very appropriate means for the manipulation of harmful or protective immunity (2-4).

The dendritic cell (DC) lineage is comprised of bone marrow-derived cells that are present in small numbers in nonlymphoid as well as lymphoid tissues (1). Nonlymphoid DC, such as epidermal Langerhans cells (LC), display an immature phenotype, i.e., they are capable of acquiring and processing ...