微生物学技术

We have found that the DNA extraction procedure of Metzenberg and Baitch (Neurospora Newsl. 28:20)/Stevens and Metzenberg (Neurospora Newsl. 29:27) while giving excellent yield and size of DNA is some ...

Preparation of fungi for DNA extraction typically involves growing cultures in liquid culture in Erlenmeyer flasks Roux bottles or even microfuge tubes (Cenis 1992 Nucl. Acids Res. 20:2380). Growing f ...

Most RNA isolation techniques currently in use have been developed for the processing of large quantities of material. These typically involve multiple phenol extractions (Reinert et al. 1981 Mol. Cel ...

We have developed a method for isolating high quality total RNA from N. crassa mycelia that reliably yields large quantities. It is possible to extract more than 50 minipreps at once. Procedures of RN ...

Molecular biology experiments often require preparation of small amounts of DNA from many samples. This abbreviated DNA isolation method yields an average of 0.6 micrograms of genomic DNA that is suit ...

Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts expensive for chemicals such as cesium chloride or suitable only for small scale preparations. W ...

-Set up 60 mm dishes of P11 cells to be 100 confluent at time of infection.-Remove medium from dishes add 0.2 to 0.5 ml virus and adsorb for 30 � 60 minutes at room temperature rocking dishes occasio ...

adapted from Cell Biology A Lab Manual second edition volume 1-Grow up P11 cells in 15 cm plates to 70 � 80% confluence.-Infect cells with a MOI (multiplicity of infection) of 1 � 10 PFU per cell (o ...

OverviewGeneral guidelines on how to grow up a culture of Acetobacter xylinum ATCC strain 53582 . Preparation of Acetobacter MediaTo prepare ∼500 ml of liquid Acetobacter media add the following: Gluc ...

MaterialsGlass culture tubes with metal caps and labels Growth medium from media room or customized Glass pipette tubes Parafilm EquipmentVortexer Fireboy or Bunsen burner Motorized pipette Micropipet ...

IntroductionTransformation is the process of introducing foreign DNA (e.g plasmids BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria ...

AbstractChIP-Chip stands for Chromatin Immunoprecipitation and chip in the sense of DNA microarray. It is a technique to determine the genome-wide binding sites of a DNA-binding protein. While the bas ...

MaterialsPCR SuperMix High Fidelity VF2 primer (5''-TGCCACCTGACGTCTAAGAA-3'') VR primer (5''-ATTACCGCCTTTGAGTGAGC-3'') Deionized sterile H2O strip of PCR tubes 2-log DNA ladder 0.8% E-Gel® from Invitr ...

OverviewThis is a method to assemble two BioBricks using the Clontech In-Fusion PCR Cloning Kit and maintains BioBrick standard formats. There are currently several BioBrick assembly standardsthat all ...

Solutions/reagents:LB broth + selective marker50% sterile glycerolTEG (25mM Tris-Cl 10mM EDTA 50mM dextrose)20 mg/ml lysozyme10% SDS4M NaOHautoclaved waterSolution 3 (3M potassium-acetate 2M acetic ac ...

IngredientsIngredients are per culture; make enough for one extra culture to allow for pipetting error). 150μL sterile 50% glycerol 1mL TEG (25mM Tris-Cl 10mM EDTA 50mM dextrose) 111μL 20mg/mL lysozym ...

BackgroundThis protocol is adapted from "Molecular Cloning: A Laboratory Manual" Second Edition Sambrook Fritsch and Maniatis. It is a quick inexpensive way to purify large numbers of plasmids (I used ...

MaterialsFor purifying plasmid DNA from Escherichia coli cells the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filt ...

plasmid miniprep All ''miniprep'' methods reported so far for the isolation of plasmid DNA involve multiple pipetting extraction centrifugation and changes of minifuge tubes. For screening large numbe ...

OverviewGeneral guidelines for the electro-transformation of Lactobacillus sake as described by Berthier et al. and as used by Alegre et al. with Lactobacillus plantarum. Materials50 ul of L.plantarum ...