生物化学技术

Identification of a Mutant Kinase/ATP Analog PairScott T. Eblen N. Vinay Kumar and Michael J. WeberDepartment of Microbiology and Cancer Center University of Virginia Health System Charlottesville VA ...

Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose ChromatographyJoseph SambrookPeter Maccallum Cancer Institute and The University of Melbourne AustraliaDavid W. RussellUniversity of Texas Southwester ...

超越HPLC 随着科学技术的进步，对液相色谱技术的要求也不断提高，单从技术角度的改进已经不行。这就需要同时从科学与技术的角度出发，或者说从理论高度对液相色谱重新认识。因此，UPLC（超高效液相色谱）概念得以提出，将HPLC的极限作为自己的起点。 在1996年，Waters公司推出Alliance HPLC时的主要目标是提高液相色谱的"精度"。当时多数公司都认为HPLC技术已经发展到极致了、而同时用 ...

仅在一定条件下酶反应速度v才和酶量成正比例。一定条件是什么？这些条件之间有无主次关系？如何选择合适的测定条件？这些都是实验室工作者在设计或选择测酶活性浓度方法时必须面对的问题。 　　首先可从理论上探讨在什么特定情况下的反应速度才可能与酶量成正比例。酶的整个反应过程可简化如下：　　式中Kp可看成为ES的解离常数.Michaclis和Menten通过推导可得出下式方程式　　此式中V为最大反应速度，对一 ...

Phosphate Assay by Suprya JaydevContributor: Suprya JayadevDate: Sep. 8 1994ReagentsAshing buffer:10 g Mg(NO3)2 100 ml EtOH1.5 N HCl stock:119.7 ml concentrated HCl (11.6M @ 36% by weight)880.3 ml H2O ...

Preparation of bOG-DOPG mixed micellesContributor: Date: November 22 1993Materials:All glassware must be acid washed rinsed thoroughly with water then rinsed with acetone and dried.Redistill acetone.R ...

Lipid analysisThin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer of adsorbent material which has b ...

Lipid analysisDuring this week you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a very sensitive method for the separa ...

Sphingomyelin Mass MeasurementContributor: Suprya JayadevDate: Oct. 20 1994Reagents:2N methanolic NaOH2N HClProtocol:Bligh & Dyer extraction1) Pellet approximately 1 X 107 cells.2) Resuspend pellet in ...

Lipoprotein Isolation and AnalysisSince lipids are not readily soluble in aqueous media they are normally transported together with protein components. The resulting lipoproteins are composed of the l ...

EXTRACTION AND ANALYSIS OF LIPIDSThe lipophorin molecule that we isolated from locust hemolymph functions as a re-usable lipid shuttle. HDLp has a density of approx. 1.08 g/ml and contains less lipids ...

Choline Labeling and TNF Treatment of HL-60 CellsContributor: Suprya JayadevDate: Aug. 6 19911) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 t ...

Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates Ronald L. Schnaar~Professor Johns Hopkins University Medical School Baltimore Maryland 21205This procedure al ...

Detection of Glycoproteins on BlotSource: Contributed by Sharad Purohit Paller's LabReagentsSodium acetate Buffer (200mM pH 5.5): Prepare a 200 mM solution of sodium acetate by disolving 12.0 gam of ...

T.H. Plummer Jr. and A.L. Tarentino Department of Biochemistry New York State Department of Health Wadsworth Center for Laboratories and Research Albany New York 12201Parameters of deglycosylation var ...

Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation Rockville Maryland 20850Flow assays allow visualization of cell adhesion under well-defined wall shear stress. T ...

Immunohistochemistry using Anti-Ganglioside AntibodiesTadashi Tai~Head Department of Tumor Immunology The Tokyo Metropolitan Institute of Medical Science Tokyo JapanAn indirect method using immuno- fl ...

Immunostaining Thin Layer Chromatograms Of GlycolipidsJohn L. Magnani~GlycoTech Corporation Rockville Maryland 20850Immunostaining thin layer chromatograms allows for very sensitive detection of funct ...

Many different molecules have been described to promote cell adhesion including several cell surface carbohydrate-binding proteins. Measuring cell adhesion in the convenient 96-well microtiter plate f ...

Coomassie Blue Stain: (for gels) 1) Combine 225 ml Methanol with 225 ml ddH2O. 2) Add 0.5 grams of Coomassie Blue. 3) Just before use add 50 ml acetic acid to 10% final concentration. 4) Stain for 2 ...