生物化学技术

Identification of a Mutant Kinase/ATP Analog PairScott T. Eblen N. Vinay Kumar and Michael J. WeberDepartment of Microbiology and Cancer Center University of Virginia Health System Charlottesville VA ...

微量热法(包括等温滴定量热和差示扫描量热)是近年来发展起来的一种研究生物热力学与生物动力学的重要结构生物学方法，它通过高灵敏度、高自动化的微量量热仪连续和准确地监测和记录一个变化过程的量热曲线，原位(insitu)、在线(on-line)和无损伤地同时提供热力学和动力学信息。 微量热法具有以下特点： 1.它不干扰蛋白质和核酸的生理功能，具有非特异性的独特优势，即对被研究蛋白质和核酸体系的溶剂性质 ...

Purification of Demethylated SphingomyelinContributor: Suprya JayadevDate: Mar. 11 1991I. Lower chloroform phase:1) Dry on rotovapor system with house vacuum lines. It is not necessary to dry sample c ...

仅在一定条件下酶反应速度v才和酶量成正比例。一定条件是什么？这些条件之间有无主次关系？如何选择合适的测定条件？这些都是实验室工作者在设计或选择测酶活性浓度方法时必须面对的问题。 　　首先可从理论上探讨在什么特定情况下的反应速度才可能与酶量成正比例。酶的整个反应过程可简化如下：　　式中Kp可看成为ES的解离常数.Michaclis和Menten通过推导可得出下式方程式　　此式中V为最大反应速度，对一 ...

Lipid analysisDuring this week you will analyze the fatty acid composition of the individual lipid fractions recovered from the TLC plate. Gas chromatography is a very sensitive method for the separa ...

Lipoprotein Isolation and AnalysisSince lipids are not readily soluble in aqueous media they are normally transported together with protein components. The resulting lipoproteins are composed of the l ...

Sphingomyelin Quantitation Post-choline Labeling of HL60 CellsContributor: Suprya JayadevDate: Jun. 16 1992ReagentsTMT buffer:0.19 M Tris-HCl (pH 7.4-7.6)12 mM MgCl20.2 % Triton X-100SMase solution:SM ...

Choline Labeling and TNF Treatment of HL-60 CellsContributor: Suprya JayadevDate: Aug. 6 19911) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 t ...

Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates Ronald L. Schnaar~Professor Johns Hopkins University Medical School Baltimore Maryland 21205This procedure al ...

T.H. Plummer Jr. and A.L. Tarentino Department of Biochemistry New York State Department of Health Wadsworth Center for Laboratories and Research Albany New York 12201Parameters of deglycosylation var ...

Dynamic Flow Assay in a Parallel Plate Flow ChamberJohn T. Patton~GlycoTech Corporation Rockville Maryland 20850Flow assays allow visualization of cell adhesion under well-defined wall shear stress. T ...

Glycolipid Binding AssaySource: Contributed by Pingsunjim Paller’s LabAbstract: This protocol can be used for the detection of glycolipids binding to immunoprecipited protein. ProcedureA: Preparation ...

Immunostaining Thin Layer Chromatograms Of GlycolipidsJohn L. Magnani~GlycoTech Corporation Rockville Maryland 20850Immunostaining thin layer chromatograms allows for very sensitive detection of funct ...

Immunohistochemistry using Anti-Ganglioside AntibodiesTadashi Tai~Head Department of Tumor Immunology The Tokyo Metropolitan Institute of Medical Science Tokyo JapanAn indirect method using immuno- fl ...

A Method for Micro-Scale Isolation and Purification of GangliosidesStephan Ladisch~Director Center for Cancer and Transplant Biology Children's National Medical Center Washington D.C. 22010This solve ...

Acetylation (or Succinylation) of Amino Groups on ProteinsREFERENCE: Hanock and Benz. 1986. BBA. 860:699-707.PURPOSE: Derivitization of amino groups to remove the positive charge characteristics of th ...

Amicon Stirred Ultrafiltration Cells (Models 8050 8400) Description - Membranes - Assembly - Operation - Limits - Sterilization - Maintenance - Troubleshooting DescriptionFor protein concentration gas ...

Liposome Swelling AssayREFERENCES: Nikaido and Roseenberg (1983) J.Bact 153: 241-252; Yoshimura et al. (1983) J.Bact 258: 2308-2314.METHOD:6.2 µmoles of lipid (ideally egg phophatidylcholine or dipalm ...

James Hardwick's angiotensin assay protocol This specific procedure was developed to assay the activity of the Lck kinase expressed from a retroviral vector in rat fibroblasts. You can obviously use f ...

Assay of superoxide dismutase activity by combining electrophoresis and densitometryAbstract. A modified technique was developed to assay superoxide dismutase (SOD) activity by combining polyacrylamid ...