Reactive oxygen species are implicated in many human diseases and aging process. Much of the evidence is based on experimental data indicating increasing rates of lipid peroxidation in disease states and the ameliorating effects of antioxidants. It is becoming increasingly evident t ...
Quantitative measurement of cellular oxidative stress (COS) and cytotoxicity are important to establish their significance in pathophysiologic conditions and disease states. So far, ample methods have been described to determine these processes based on spectrophotomet ...
Studies have demonstrated that plasma nitrite () reflects endothelial nitric oxide (NO) production. In addition, has been shown to have biological activities associated with its reduction to NO in blood and tissues. Therefore, determination of plasma has been proposed as a prognostic ma ...
The partial pressure of oxygen (pO2) plays a determining role in the energy metabolism of aerobic cells. However, low pO2 level induces pathophysiological conditions such as tumor hypoxia, ischemia or reperfusion injury, and delayed/altered wound healing. Especially, pO2 level in the t ...
Free radicals are highly reactive compounds that play an essential role in many biological processes, both beneficial and deleterious. Detection and quantification of these species is critical to develop a better understanding of normal and pathophysiological functions at the ce ...
Phenolics are structurally assorted and are generally part of a complex mixture isolated from plant and biological origin matrices. A wide gamut of natural products have been the focus of main study for phenolic compounds while urine and blood are the two main biological fluids that have been an ...
Ample evidence supports the critical role of oxidized low-density lipoprotein (ox-LDL) in initiation and progression of atherosclerosis. Oxidation of LDL is a complex process involving several steps (processes) of reactions such as initiation and propagation. Both proteins and l ...
Antioxidants have been found to be effective as prophylatic and therapeutic agents for different diseases such as diabetes, cancer, and neurodegenerative disorders. However, antioxidant substances can present poor solubility in water, inefficient permeability, gastroin ...
Current therapies for Alzheimer disease (AD) such as the acetylcholinesterase inhibitors and the latest NMDA receptor inhibitor, Namenda, provide moderate symptomatic delay at various stages of the disease, but do not arrest the disease progression or bring in meaningful remissio ...
With the recognition that chronic exposure to oxidative stress occurs in many disease states and can be deleterious to the human health, great interest has emerged in understanding the mechanisms by which mammalian cells develop resistance to chronic oxidative stress. In order to study the ...
Protein drugs, such as recombinant enzymes useful for detoxification and replacement therapies, have extraordinary specificity and potency. However, inherently inadequate delivery to target sites and rapid inactivation limit their medical utility. Using chaperone poly ...
Lipid raft-associated cholesterol has been identified as a pivotal player among membrane lipids in regulating cellular functions. Cholesterol of the vascular endothelial cell (EC) membranes is also being recognized as an important element in the vascular EC signaling. However, me ...
Erectile dysfunction (ED) is defined as the inability to attain and/or maintain penile erection sufficient for satisfactory sexual performance. Oxidative stress has been demonstrated to be involved in the pathophysiology of age- or diabetes-related ED. Superoxide dismutase (SO ...
Most mammalian cells cannot synthesize vitamin C, or ascorbic acid, and thus must have efficient mechanisms for its intracellular recycling. Ascorbate can be recycled from both its oxidized forms using electrons from several intracellular reducing co-factors, including GSH and the ...
Oxidative stress is fast becoming the nutritional and medical buzzword for the twenty-first century. The theoretical importance of oxidative stress in diabetes is highlighted by its potential double impact on metabolic dysfunction on one hand and the vascular system on the other hand. T ...
Two-dimensional blue native (2D BN)/SDS-PAGE is the method of choice for the global analysis of the subunits of complexes in membrane proteomes. In the 1st dimension complexes are separated by BN-PAGE, and in the 2nd dimension their subunits are resolved by SDS-PAGE. The currently available pr ...
A large number of Gram negative pathogens use a specialized needle-like molecular machine known as Type III Secretion (T3S) system. This highly sophisticated molecular device consists of a basal body spanning the two bacterial membranes and a protruding needle structure that is connec ...
A method is described for the preparation and usage of an E. coli cell-free translation system primed to incorporate the commercially available photoreactive analogue of phenyalanine, pBpa, into newly synthesized proteins. Incorporation is achieved by means of an amber suppressor t ...
The Tat system is a protein export system dedicated to the transport of folded proteins across the prokaryotic cytoplasmic membrane and the thylakoid membrane of plant chloroplasts. Proteins are targeted for export by the Tat system via N-terminal signal peptides harbouring an S-R-R-x-F ...
Site-directed spin-labeling and the analysis of proteins by electron paramagnetic resonance spectroscopy provides a powerful tool for identifying sites of contact within protein complexes at the resolution of aminoacyl side chains. Here we describe the method as we have used it to stu ...
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