生物化学技术

Connective Tissue Stain for Muscle Biopsy :GOMORI TRICHROMESECTIONSUnfixed cryostat or cold Formol calcium/gum sucrose fixed sections on muscle biopsy.SOLUTIONSStaining solutionChromotrope 2R 0.6gFast green FCF 0.3gPhosphotungstic acid 0.6gGlacial acetic acid 1 ...

半乳糖苷酶和碱性磷酸酶染色方法Histochemical StainingThis protocol has been optimized for b-galactosidase and human placental alkaline phosphatase staining in retinal tissue and cultured cells.Solutions0.5% Gluteraldehyde/PBS25 ml 1X PBS0.5 ml 25% gluteraldehyde (Sigma)20% Pa ...

精子顶体染色 Stain for Sperm Acrosomes 1. After sperm preparation, adjust sperm concentration as high as possible (5-10 x 106/ml). 2. Prepare a smear of sperm suspention onto a slide and allow it to dry. 3. Place slides in 0.1% (w/v) naphthol yellow S in 1% (v/v) aqueous acetic acid for 30 min. 4. Blot the slides dry and then rinse t ...

Antigen retrieval for immunohistochemistry with paraffin-embedded tissues免疫组化石蜡包埋组织抗原修复BackgroundTissue sectioning with paraffin embedded tissues offers several advantages as compared to work with frozen tissues including ease and the fact that microtomes are ...

酶法、热修复、微波法修复抗原 Enzymatic antigen retrieval ，Heat Mediated Antigen Retrieval，Microwave Method Formalin fixed tissue requires an antigen retrieval step before immunohistochemical staining can proceed. This is due to the formation of methylene bridges during fixation, wh ...

高温处理＋柠檬酸钠或EDTA修复石蜡包埋样品抗原 High Temperature Antigen Unmasking Technique using Sodium Citrate or EDTA Buffer 1. Cut and mount sections on slides coated with a suitable tissue adhesive.2. Deparaffinise sections and rehydrate to distilled water.3. Place sections in 0.5% hydrogen per ...

胰酶消化修复抗原 Trypsin Digestion of Paraffin sections prior to Immunohistochemistry 1. Preheat the following to 37oC using a water bath:(i) 200ml of TBS(ii) 200ml of distilled water.2. Dissolve 0.2g Trypsin 250 and 0.2g Calcium Chloride in the 200ml of TBS.3. Once the trypsin solution is at 37oC, pH to 7.8 with ...

Preparation and use of Zamboni’s-fixed Frozen Sections in ImmunohistochemistryZAMBONI’S FIXATIVE (PARAFORMALDEHYDE/PICRIC ACID)SUGGESTED PREPARATION METHOD1. Mix 20g paraformaldehyde with 150ml double-filtered, saturated aqueous picric acid.2. Heat to 60oC in f ...

高温处理＋胰酶消化修复抗原 High Temperature Antigen Unmasking Technique followed by Trypsin Digestion 1. Cut and mount sections on slides coated with a suitable tissue adhesive.2. Deparaffinise sections and rehydrate to distilled water.3. Place sections in 0.5% hydrogen peroxide/methanol ...

Specimen preparation for electron microscopy非常详细的电镜样品制备方法E.M. PROCESSING SCHEDULE - EPOXY RESIN Fix tissue in 2.5% glutaraldehyde in 0.1M sodium cacodylate buffer at 4oC, for a minimum of 4 hours. Tissue should be cut into approx. 1mm cubes for fixing. This may be done in a drop of fix on a sheet of dental ...

Histological fixatives 组织固定剂和固定方法Fixation: Confers chemical stability on the tissue Hardens the tissue (helps further handling) Halts enzyme autolysis Halts bacterial putrefaction May enhance later staining techniques Introduces a 'consistent artefact' Classifi ...

Preparation of tissue section adhesives 介绍3种样品不易脱落方法处理玻片SECTION ADHESIVES It is advisable to use an adhesive to promote tissue attachment to the glass slides used in histological preparation. This is routinely achieved by the application of a smear of glycerin/albumen mixture to t ...

Fixation: Specimens may be routinely fixed in 10% neutral buffered formalin (NBF). Fixation should be commenced as soon after surgical excision as is practically possible to prevent autolysis and bacterial decomposition. Formalin fixation should ideally be allowed to proceed for at l ...

C. elegans whole mount immunohistochemistry with Bouin's FixativeI have developed a fixation protocol which provides an alternative set of conditions to test antisera that work poorly on paraformaldehyde fixed worms (e.g. the Finney protocol). A Bouin's fixative of 75 ml saturated pic ...

Cryotomy 详细介绍冷冻切片的方法All tissue types except muscle:Tissue should preferably be snap-frozen by quenching in liquid nitrogen. Quenching is accomplished by putting a small amount of OCT compound onto a cork disc, placing the freshly biopsied tissue onto that and then dropping the disc ups ...

药品名称：甲氨喋呤 药物别名：MTX 药物类别：抗肿瘤抗代谢类 适应症：用于急性白血病，尤其是急性淋巴细胞性白血病，绒毛膜上皮癌及恶性葡萄胎等效果较好。对头颈部肿瘤、乳腺癌，舌咽癌，膀胱癌，睾丸肿瘤，肺癌及盆腔肿瘤均有一定疗效。 作用机理：主要通过对二氢叶酸还原酶的抑制而达到阻碍肿瘤细胞DNA的合成，而抑制肿瘤细胞的生长与繁殖。MTX 对二氢叶酸还原酶具有高度的亲和力，与其结合后阻止二氢叶酸还原酶为四氢叶酸，后者在嘌呤，嘧啶核苷酸生物 ...

小鼠的组织学技术--组织处理，染色Histology of mouse tissues(M. Fero)Choice of fixatives:Fresh tissues should be immediately placed in plastic histology cassettes and immersed in an appropriate fixative to avoid tissue autolysis. Intestine, liver, and brain are particularly prone to autoly ...

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　CD44是一种复杂的分子聚合体，与很多炎症和肿瘤疾病相关。致力于解答这种细胞表面粘连分子的作用的研究此起彼伏，相关结果也不断被公布。然而，研究人员所观察到的CD44的作用机制不尽相同。但是，各国研究人员普遍认为，CD44最终将成为炎性疾病治疗的一个目标。　　CD44与炎症反应相关的证据究竟是什么呢？　　在炎症的初始阶段，炎性细胞聚集到感染或损坏部位。在这里，炎性细胞和局部的实质细胞都会释放炎性介质，原发损害将被处理，通常炎 ...

对CD44的研究时间较长、较深入亦较广泛。已知其为分布极为广泛的细胞表面跨膜糖蛋白，主要参与异质性粘附，即肿瘤细胞与宿主细胞和宿主基质的粘附，异质性粘附在肿瘤细胞侵袭转移中起促进作用。人类CD44基因位于11号染色体短臂，全长约50kb，共由20个高度保守的外显子组成，每个外显子长度70-210bp不等，中间由长短不一的内含子分隔。CD44基因的20个外显子按照转录方式不同可分为组成型外显子（C）和变异型拼接外 ...