生物化学技术

Surface plasmon resonance (SPR) employs the optical principle of SPR to measure changes in mass on a sensor chip surface in real time. Surface chemistry has been developed which enables the immobilization of lipid bilayers and determination of protein–membrane interactions in real time. ...

Surface analytical tools as surface plasmon resonance (SPR) have become increasingly important in biomedical research since they offer high detection sensitivity compared to traditional biomedical methods. For the use of SPR as a biomedical research tool there is a need to immobilize ...

The G-protein coupled receptor rhodopsin is a classical example of a seven transmembrane helix receptor; it is photoexcited and transmits this light signal to a G-protein mediated cascade. Many components of this receptor-triggered cascade can be purified in their native forms from nat ...

Surface plasmon resonance is widely used to study binding interactions with proteins, potentially yielding information on kinetics, thermodynamics and active concentrations. However, the technology cannot identify the involved interaction partners. Mass spectromet ...

The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) creates a comprehensive protein investigation approach wherein SPR is employed for protein quantification and MS is utilized to structurally characterize the proteins. In such, MS utterly complements ...

Surface plasmon resonance (SPR) is one of the leading tools in biomedical research. The challenge in its use is the controlled positioning of one of the components of an interaction on a carefully designed surface. Many attempts in interaction analysis fail due to the non-functional or unsucce ...

Surface plasmon resonance (SPR) analysis is rather unique in that it allows assay of binding constants (affinity) and kinetic analysis of binding phenomena. This introductory chapter deals with some specific features that are relevant to many diverse applications. The role and impact of ...

This chapter presents an introduction to the kinetic analysis of SPR biosensor data for the determination of affinity and kinetic rate constants of biomolecular interactions between an immobilized and a soluble binding partner. The need to be aware of and critically test the assumptions ...

Surface plasmon resonance (SPR) is a highly sensitive method for the detection of molecular interactions. One interacting partner is immobilized on the sensor chip surface while the other is injected across the sensor surface. This chapter focuses on high-affinity immobilization of p ...

Surface plasmon resonance (SPR) is a robust method to detect and quantify macromolecular interactions; however, to measure binding interactions, one component must be immobilized on a sensor surface. This is typically achieved using covalent immobilization via free amines or thio ...

Direct assay of small molecules by SPR in general is troublesome and at least tedious procedures have to be applied. Competition experiments offer an attractive alternative. A small ligand known to bind to the analyte is immobilized on an SPR sensor surface, and the binding of the larger analyte in t ...

Sensitive detection of small molecules using surface plasmon resonance (SPR) presents significant challenges as the antigen cannot serve as a signal generator because of its low mass; efficient binding of the target requires the binding event to be spaced from the sensor surface through a s ...

Prion diseases, also called transmissible spongiform encephalopathies (TSEs), are a group of neurodegenerative disorders affecting animals and humans. No effective treatments are currently available for the diseases, vCJD in particular. It is believed that the formation of pro ...

We have designed a novel high-throughput (HTP) kinase assay using an array-based surface plasmon resonance (SPR) apparatus. For high flexibility and performance, the kinase assay procedure is divided into an in vitro phosphorylation part and a phospho-detection part on a sensor chip. Not o ...

Linkage of proteins to carbon nanotubes (CNTs) is fundamentally important for applications of CNTs in medicinal and biological fields, as well as in biosensor or chemically modulated nanoelectronic devices. In this contribution, we provide a detailed protocol for the synthesis and ch ...

A method for the non-covalent attachment of proteins to single-walled carbon nanotubes (SWNTs) is described. In this method, the protein is adsorbed to SWNTs that are suspended using sodium cholate, a surfactant and bile salt. The sodium cholate is then removed by dialysis with retention of the p ...

In this chapter, a multi-step protocol for covalently linking functionalized multi-walled carbon nanotubes (MWCNT) to deoxyribonucleic acid (DNA) oligonucleotides is provided. X-ray photoelectron spectroscopy (XPS) is used to characterize the initially formed amine-te ...

Fluorescently labeled carbon nanotube probes (CNTP) are prepared by derivatizing oxidized (o)-MWNTs with a fluorescein dye. Capillary electrophoresis coupled with laser-induced fluorescence (CE-LIF) detection is used to separate and detect CNTP in multidrug-resistant ce ...

Cell trafficking of carbon nanotubes (CNTs) is an area of scientific inquiry that has great implications in medicine, biosensing, and environmental science and engineering. The essence of this inquiry resides in the interaction of carbon nanostructures and cell membranes, regulat ...

The field of carbon nanotube (CNT) functionalisation is increasingly growing for the purpose of enhancing the biocompatibility of CNT for medical and biological applications. Properties of CNT such as the type of functionalisation, charge density, and the dispersibility profile a ...