蛋白质技术

Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extraction followed by methanolic ammonium acetate precipitation - an effective protocol for sample preparation from protein-po ...

David M. SchieltzandMichael P. Washburn This protocol was adapted from "The Use of Mass Spectrometry in Proteomics" Chapter 8 inProteins and Proteomics(ed. Simpson). Cold Spring Harbor Laboratory ...

Lightning-Link 30 seconds hands on Very Easy to use 100% Antibody Recovery No separation steps Little antibody needed Easy to Scale Up Over 40 available labels http://www.youtube.com/v/w0XmU9Niwns& ...

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Methylene Blue DNA staining protocolProtocol: Load 2-5X the amount of DNA that would give bands of moderate intensity on an ethidium bromide stained gel. Typically this is something on the order of 0. ...

1. Prepare 50X TAE as:242 g Tris Base57.1 mL Glacial Acetic Acid100 mL 500 mM EDTA pH 8.0600 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Mix the following:0.40 g Agarose4.00 mL 50X TBE (4X TAE fina ...

Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed separating complex mixtures of DNA into different sized fragments by electrophoresis was a well-established te ...

Manipulating and analyzing DNA are fundamentals in the field of molecular biology. Indeed separating complex mixtures of DNA into different sized fragments by electrophoresis was a well-established te ...

Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially ...

ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS:Use 0.8% agarose (w/v) for high molecular weight DNA fragments and 1 - 1.2% for smaller DNA fragments. 0.5% gels are very flims ...

Purpose:Denaturing gradient gels are used to detect non-RFLP polymorphisms. The small (200-700 bp) genomic restriction fragments are run on a low to high denaturant gradient acrylamide gel; initially ...

ELECTROPHORESIS OF DNA IN AGAROSE GELSA). AGAROSE CONCENTRATIONS:Use 0.8% agarose (w/v) for high molecular weight DNA fragments and 1 - 1.2% for smaller DNA fragments. 0.5% gels are very flims ...

What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively charged molecule and is moved by electric curren ...

What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. DNA is a negatively charged molecule and is moved by electric curren ...

What is Electrophoresis? Electrophoresis is a technique used in the laboratory that results in the separation of charged molecules. In this CyberLab we are separating molecules of DNA that we got from ...

Capillary electrophoresis is a very sensitive analytical technique. Sample components are separated within a fused silica capillary using one of several modes of electrophoresis. The technique can be ...

NuPAGE GelsA gel electrophoresis system used for SDS-PAGE protein analysis. The gels are made up of Bis-Tris-HCl (pH 6.4) polyacrylamide and are intended for denaturing conditions only.NuPAGE Electrop ...

1. Prepare 20X TBE as:216 g Tris Base110 g Boric Acid80 mL 500 mM EDTA pH 8.0700 mL ddH2OMix. Bring volume to 1 L. Autoclave.2. Prepare Acrylamide solution as:6 % Acrylamide 60.0 g Acrylamide0.25 % Bi ...

Acrylamide Urea Gel (35 ml)10%15%40/2% acrylamide 10 ml 13.1 ml10X TBE 3.5 ml3.5 mlUrea 15 g15 gH20 10ml7.0 mlMicrowave ~10 seconds and stir until dissolvedDegas 1-2 minAdd 0.3 ml 10% ammonium persu ...