RNA实验技术

siRNA Database Searchable database of Silencer ™ Validated and Pre-designed siRNAs to 34000 human mouse and rat targets. All siRNAs in the database have been designed for maximum potency and specifici ...

The use of small interfering RNAs (siRNAs) to induce targeted gene silencing in mammalian cells offers researchers a powerful tool for analyzing gene function. Ideally one would like to work with indi ...

N.B: Gloves should be worn at all times during preparation of in vitro RNA. All solutions should be RNase-free i.e. made with DEPC water if home-made or bought specifically to use for RNA work. You wi ...

1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg)7.8 ml DDW5 ml 5X transcription buffer ( stratagene )1 ml 750 mM DTT1 ml Rnasin (RNase inhibitor; 15U/ml)1 ml 10 mM ATP (pH 7)1 ml 10 mM CTP ...

Polymerase III in vitro Transcription Steve HahnFor the following reactions use appropriate shielding and dispose of radioactive waste properly!A 20 microliter transcription reaction contains:4.0 μ ...

Run-on transcription monitors the regulation of transcription in isolated nuclei.A. Preparation of Nuclei - (do everything at 0℃ to 4℃ in 50 ml tubes)1. Pellet between 30 and 300 million cells at 1500 ...

Transcription of cRNA probeReagents/SolutionsDNA (PCR or plasmid) with bacteriophage RNA polymerase promoter in suitable orientation (see note 1 ) ~1µg/µl in TE buffer 10mM MgCl2 5x Transcription Buff ...

The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3.A 5-primer with ...

RNA extraction buffer:0.1M NaCl2% SDS50mM Tris/HCl (pH9)10mM EDTA20mM ß-mercaptoethanol1. Grind leaf tissue on liquid N in mortar and pestle.2. Transfer the ground tissue to a 10ml conical bottom cent ...

T℃OS-M6 cells grow in 6-well plates 2ml media total T℃V1PD cells grow in 100mm plates.1: Aspirate off the media from the cells and was the cell monolayers with 2x5ml (To 100mm dish) or 2x1ml (to 6-wel ...

Based on: Wan C.-Y. and Wilkins T.A. 1994. Anal. Bioch. 223:7-12. 1.Collect young expanding leaves (or other tissue) and freeze in liquid N2 and store at -80℃. 2.Pulverize tissue to a fine powder in a ...

Chemicals needed:Glucose L-( )-Arabinose Sigma Cat # A3256 L-Rhamnose Sigma cat # R3875 Cb (carbenicillin) Sigma cat # 1389DL-p-Chlorophenylalanine Sigma cat # C6506Media Recipes:YEG recombination pla ...

RNA interference (RNAi) is a biological process in which the introduction of double-stranded RNA (dsRNA) into a cell results in targeted post-transcriptional gene silencing.Historically RNAi has been ...

RNA interference (RNAi ) by double stranded RNA (dsRNAs) molecules of approximately 20-25 nucleotides termed short interfering (siRNAs) is a powerful method for preventing the expression of a particul ...

Chemicals needed:Glucose L-( )-Arabinose Sigma Cat # A3256 L-Rhamnose Sigma cat # R3875 Cb (carbenicillin) Sigma cat # 1389DL-p-Chlorophenylalanine Sigma cat # C6506Media Recipes:YEG recombination pl ...

We use the Promega Ribomax Large Scale RNA Production System T7 (Cat. No.: P1300) to produce dsRNAs and the RNAi Exp.html" target=_blank Jack Dixon protocol ( RNAi _Dixon.html" target=_blankdownloaded ...

The Easiest Route to Guaranteed SilencingIncreasing numbers of researchers are using small interfering RNAs (siRNAs) to reduce the expression of specific mammalian genes. Applications of siRNA induced ...

在人细胞中快速、高水平地表达目的蛋白 在2至3个星期内即可获得高效价的病毒 无需噬斑纯化 靶细胞可以是分裂或不分裂的细胞（dividing/or nondividing cell） CLONTECH的Adeno-X™表达系统是一个特别 高效的腺病毒表达系统，用于在人细胞中瞬时及高水平地表达目的蛋白质。由于Adeno-X™病毒DNA中含有特别设计的克隆位点，你可以在2星期内获得高效价的腺病毒 ...

Restriction Map and Cloning Site of the RNAi-Ready pSIREN-DNR Vector. Unique restriction sites are in bold. RNAi-Ready pSIREN-DNR is provided as a linearized vector digested with BamH I and EcoR I. N ...

RNA Marker ( RL1000; RL6000; RL10000) 可以进行一般琼脂糖凝胶电泳和变性琼脂糖凝胶电泳。以RL10000为例，具体使用方法如下：普通琼脂糖凝胶电泳时1.按下列组份配置RNA Marker样品。2.均匀混合后65℃加热10分钟，迅速冷却至室温（最好用PCR仪)。3.使用高质量的琼脂糖，用1×TAE Buffer制备3%凝胶，制胶时凝胶中请加入溴乙锭(最终浓度：1 ...