DNA实验技术

锐博生物新的EdU荧光标记技术，能够方便、快速、准确的检测研究细胞的增殖、周期、凋亡、活性、分化、迁移及示踪。详细使用说明请见下面的PDF文档！C10310&C10312 Cell-Light TM EdU荧光显微镜检测试剂盒说明书.pdf需要订购相关产品请填好下面的合同和信息卡发到：sales-cd@ribobio.com！EdU&EU 订购信息卡.xlsRibobio 2010年购销合 ...

锐博生物新的EdU荧光标记技术，能够方便、快速、准确的检测研究细胞的增殖、周期、凋亡、活性、分化、迁移及示踪。详细使用说明请见下面的PDF文档！C10311 Cell-Light TM EdU 流式细胞仪 检测说明书.pdf需要订购相关产品请填好下面的合同和信息卡发到：sales-cd@ribobio.com！EdU&EU 订购信息卡.xlsRibobio 2010年购销合同.doc ...

mRNA-Seq_SamplePrep_1004898_D.pdf

Small RNA Sequencing Sample Preparation Guide.pdf

Preparing of cell extracts ...

DNA and RNA EXTRACTIONS A protocol / method / schedule /procedure for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves (See also DNA Isolation protocol) ...

PlasmidsA DNA molecule can be amplified using the in vitro technique of PCR to obtain large number of identical molecules. Such pure DNA is needed for example to sequence it to use as a probe in South ...

PlasmidsA DNA molecule can be amplified using the in vitro technique of PCR to obtain large number of identical molecules. Such pure DNA is needed for example to sequence it to use as a probe in South ...

ProcedurePurify the PCR product. Before adding the overhangs it is very important to remove all the Proofreading DNA Polymerase (Pfu) by purifying the PCR product carefully (e.g. with a commercial PCR ...

After amplification with a proofreading polymerase place samples on ice and add 0.7-1 unit of Taq polymerase per tube directly into the PCR reaction tube. Mix well. Incubate at 72°C for 8-10 minutes. ...

This protocol uses Promega''s pGEM-T kit (#A3600).PCRFor TA cloning it is optimal if the PCR primers have G''s at the 5'' end as this will maximize the probability of Taq polymerase adding the termina ...

Here is some informal background information that will hopefully provide support and context if you want to use ET recombination. Perhaps needless to mention this information has not been refereed is ...

AbstractFunctional genomics require manipulation and modification of large fragments of the genome. Such manipulation has only recently become more efficient due to the discovery of different techniqu ...

A stab culture is made by inoculating bacteria into a vial containing LB agar with the appropriate antibiotic. After overnight incubation bacterial growth should be visible both in the puncture and on ...

Proteinase K digestion Mix DNA extraction buffer 98 μl ReagentB 2 μl ProteinaseK Mix fresh. 100 μl is enough for a small pea size chunk of tissue or one embryo Place small piece of tissue or embryo in ...

Material100% ethanol (for analysis) sodium acetate -20°C freezer Optional: you can use linear polyacrylamide glycogen or tRNA as precipitation carrier --zurdo 03:45 9 October 2009 (EDT) tabletop centr ...

PREPARE SOLUTIONS 1. 10X Dephosphorylation buffer:0.5 M Tris-HCl pH 8.5 1 mM EDTA PROCEDURENOTE: 1 unit of Alkaline Phosphatase (AP) can hydrolyze 50 pmol of 5'' terminal phosphorylated DNA fragment ...

INTRODUCTION High-throughput whole-genome analysis has become a practical and important technique to understand nuclear processes such as transcription replication and genome structure. Though microar ...

操作步骤：（实验前请先阅读注意事项）提示：第一次使用前请先在漂洗液RW瓶加入指定量无水乙醇!操作前在裂解液RLT中加入β-巯基乙醇至终浓度1%，如1 ml RLT 中加入10μl β-巯基乙醇。此裂解液最好现用现配。配好的RLT 4℃可放置一个月。1.直接研磨法（推荐）:a. 新鲜植物组织称重后取100mg迅速剪成小块放入研钵（冰冻保存或者液氮保存样品可直接称重后取100mg放入研钵 ...

DL5000 DNA Marker.doc