DNA实验技术

Mutagenesis is a fundamentally important DNA technology which seeks to change the base sequence of DNA and test its effect on gene or DNA function. The mutagenesis can be conducted in vivo (in studies ...

After an aliquot of the PCR mixture is analyzed on an agarose gel the remainder of the reaction is concentrated by ethanol precipitation resuspended in buffer and subjected to a simultaneous fi ...

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The gel shift or electrophoretic mobility shift assay provides a simple and rapid method for detecting DNA -binding proteins. This method has been used widely in the study of sequence-specific DNA -bi ...

Restriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restriction enzyme in its respective buffer as recommended by the supplier and at ...

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helvetica" size="2"Use of oligonucleotides in various research applications requires certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your ...

随着基因工程的发展，常常需要构建一种能高水平表达异源蛋白质的表达载体。启动子对外源基因的表达水平影响很大，是基因工程表达载体的重要元件。因此研究启动子的克隆方法，对研究基因表达调控和构建表达载体至关重要。 迄今为止，国外尚未见到有关启动子克隆方法的综述性报道，国内仅孙晓红等曾就启动子的结构、分类、克隆方法和食用菌中已经分离到的启动子作过综述。而近年来又有许多改进的克隆启动子的方法获得了多方面的成功 ...

This is a rapid method for chemical DNA sequencing which is commonly used as ladder for footprinting reactions or for sequencing of short DNA oligonucleotides. Reference: Bencini et al. (1984) Biotech ...

近10年来，现代分子生物学技术越来越广泛地被用于人类疾病研究的诸领域，为了解病理状态下基因组DNA 的变化积累了新资料。目前认为，人类基因组并非人们想像的那样稳定，诸如基因重排、扩增、缺失，突变和DNA 甲基化类型改变等时有发生，这些改变对于基因表达和调控，以及疾病过程的发展与转归等方面均具有重要意义。 医院病理科档案中积存的大量石蜡包埋组织，是一个可靠的分子生物学研究的材料来源。 ...

DNA methylation is an epigenetic event that affects cell function by altering gene expression and refers to the covalent addition of a methyl group catalyzed by DNA methyltransferase (DNMT) to the 5-c ...

DNA 片段的克隆需要合适的载体，载体或是质粒，或是噬菌体，或是病毒，通常大多经过人工改造一、质粒 常用的有pBR322，pUC系列质粒等。 （一）pBR322质粒是4362bp的环状双链DNA 载体，有2个抗药性基因（四环素和氨苄青霉素），一个复制起始点和多个用于克隆 的限制酶切点。当缺失抗药性基因的大肠杆菌被pBR322成功地转化时，它便从该质粒获得了抗生素抗性。两个抗生素基因中均含供插入外源 ...

For use with GibcoBRL Random Primer DNA labeling system. Objective: To produce radioactively labeled DNA strands for the detection of target DNA or RNA sequences in various applications including So ...

动物样品的采集及处理方法 遗传学数据在野生动物和圈养动物的保护和管理中变得日益重要。在本文中，我们总结了一些能简便而有效地获得样品及数据的方法。 我们在采集样品之前，最好对将要进行的遗传学分析有所了解。然而，采集者并非都是研究者，当采集地与实验室有相当距离时，采集者常常要保存好样品直到有合适的接受者或存放地，这就要求采集者应具备一定的经验。 首先，所有样 ...

一、 DNA 酶切反应 1、 将清洁干燥并经灭菌的eppendorf管(最好0.5ml)编号用微量移液枪分别加入DNA 1μg和相应的限制性内切酶反应10×缓冲液2μl再加入重蒸水使总体积为19μl将管内溶液混匀后加入1μl酶液用手指轻弹管壁使溶液混匀也可用微量离心机甩一下使溶液集中在管底。此步操作是整个实验成败的关键要防止错加，漏加。使用限制性 ...

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A. Culture Growth: 1.For BAC isolation for shotgun library construction: Pick a smear of colonies and inoculate 3 ml TB medium containing the appropriate antibiotic. Grow the culture for 8 hours 37 ...

Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute http://www.fhcrc.org/science/labs/hahn/methods/mol_bio_meth/Big%20Dye%20Protocol.pdf ...

The following protocol is designed for subcloning inserts (I) from one vector into another vector (V). The inserts can be anywhere from 30 bp to 8 kb (possibly higher). Perform restriction diges ...

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