DNA实验技术

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Media Need LB plates (9 cm or 14 cm)to plate the library and do secondary screens.I typically screen about 500000-1 million plaques for a primary cDNA screen.This fits on 11-22 14 cm plates.I do the s ...

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基因工程：有目的的通过分子克隆技术，人为的操作改造基因，改变生物遗传性状的系列过程。 载体：能在连接酶的作用下和外源DNA片段连接并运送DNA分子进入受体细胞的DNA分子。 转化：指质粒DNA或以它为载体构建的重组DNA导入细菌的过程。 感染：以噬菌体、粘性质粒和真核细胞病毒为载体的重组DNA分子，在体外经过包装成具有感染能力的病毒或噬菌体颗粒，才能感染适当的细胞，并在细胞内扩增。 转导：指以噬菌 ...

This protocol gives M13 quality sequence when used with the Quick DNA Plasmid Prep. protocol D.2.1、Solutions Denaturation Solution　　2 M NaOH 200 ml 10N NaOH　　2 mM EDTA 4 ml 0.5 M EDTA pH 8.0　　 ...

Restriction enzymes are expensive ($40 to $200 a vial): keep the enzymes at -20℃. Plan your digests to be small and convenient. The digest is composed of DNA sample (volume should not be more than abo ...

1. Add an equal volume (equal to sample volume) of P/C to sample. 2. Mix (shake don't vortex). 3. Take aqueous (upper) layer. (If dirty sample repeat Ph/Chl step until interface is fairly clean). 4. A ...

Phenol (removes protein) 1、add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol)2、vortex3、spin 2 minutes at 12000 rpm 4°C4、transfer supernatant to a fresh tube (avoi ...

1. For double-stranded DNA templates: a. Denature template: 10ml DNA (5-10g or alkaline lysis mini prepDNA) 8ml ddH2O　　2ml 2N NaOH　　-incubate 30' at 37℃. b. Dry-ice precipitate: +10ml 4M NH4OAc 100ml ...

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琼脂糖凝胶层析的技术数据 琼脂糖凝胶作为核酸检测主要也是理想材料，使用十分广泛，其理化性质一些参数如下表： （琼脂糖是琼脂内非离子型的组分，它在 0 ~ 4 ℃， pH 4~9 范围内是稳定的。） 名称、型号 ...

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