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文献和实验Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
, however, with much improved sensitivity allowing investigation of a greater range of cell types. In the early 1980s , the “Null Point method” was introduced (4 ), which involved addition of a metallochromic calcium indicator (Arsenazo III) to cells permeabilized
Assessment of Intracellular Calcium in Cerebral Artery Myocytes
frequency and elevated global [Ca2+ ]i in cerebral artery myocytes using a rabbit model of subarachnoid hemorrhage. Here, we describe and discuss laboratory methods and procedures using the fluorescent Ca2+ indicator dyes fura-2 and fluo-4 to assess global
Calcien Blue 370 435 Calcium Green 505 532 Calcium Orange 549 576 Calcofluor RW Solution 370 440 Calcofluor White 440 500-520 Calcofluor WhiteABT Solution 380 475 Calcofluor WhiteStandard Solution 365 435 5-(and 6-)carboxySNARF-1 indicator 548(low
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