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- Vectorlabs
- SA-5004-1
- 美国
- 2026年02月27日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
有货
- 英文名：
HORSERADISH PEROXIDASE STREPTAVIDIN
- 保质期：
包装说明
- 供应商：
欣博盛生物
- 保存条件：
包装说明
- 规格：
1mg
产品名称：Streptavidin, Peroxidase (Concentrate, for IHC), 1 mg
中文名：链霉亲和素，HRP标记
品牌： Vectorlabs
产品货号： SA-5004-1
规格： 1 mg
Horseradish Peroxidase Streptavidin (HRP Streptavidin) is produced by our own coupling procedure that preserves the high specific activity of the peroxidase. Using biotinylated primary or secondary intermediates and peroxidase-labeled streptavidin, antigens can be localized in histological sections, cytospin preparations, or smears.
Features:
• Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins, nucleic acids, and other macromolecules
• Horseradish Peroxidase Streptavidin is offered as a concentrate (1 mg/ml) or in a ready-to-use (R.T.U.), prediluted, stabilized solution in a dropper bottleFor horseradish peroxidase streptavidin (Sav-HRP) that is optimized for use in solid-phase assays such as ELISAs and blotting applications, see the following product:
Streptavidin, Horseradish Peroxidase (Sav-HRP), Concentrate, for ELISAs and Blots
Detection Enzyme
Peroxidase
Format
Concentrate
Formulation
0.05 M HEPES, 0.1M Na2S04pH 6.8, preservative
Unit Size
1 mg
Storage Instructions
2-8 °C, DO NOT FREEZE
Usage Summary
Molar ratio active conjugate: 1.5. Do not use sodium azide with this product since it is an inhibitor of horseradish peroxidase. The recommended concentration range for use is 2-5 µg/ml.
Applications
Immunohistochemistry / Immunocytochemistry, In situ hybridization
Concentration
1.0 mg/ml active conjugate
Conjugate
Peroxidase
Vector Laboratories位于旧金山，由Jim Whitehead博士于1976年创立，公司致力为全球研究人员提供高性能的产品。Vectorlabs是研发免疫组织化学、免疫荧光、糖生物学和生物偶联的标记和检测试剂的先驱，并已成为该领域市场的leader。作为第一家开发出用于免疫组织化学染色的avidin-biotin酶复合物试剂盒(VECTASTAIN ABC试剂盒)和用于免疫荧光染色的防荧光淬灭封片剂(VECTASHIELD® Mounting Media)的商业化公司，Vectorlabs已经从世界各地引进了600多种用于疾病和治疗研究的试剂和试剂盒。Vectorlabs的设施通过了ISO 9001:2015认证，其研究成果已被350,000多种科学出版物引用。
Vectorlabs的产品包括VECTASTAIN ABC(Avidin-Biotin Complex Kits)、封片剂、凝集素、 酶底物、Enzyme Polymer Detection Systems、偶联试剂及试剂盒、免疫组化笔/封闭试剂/复染试剂等产品。订购咨询Vectorlabs产品请联系中国独家代理商-欣博盛生物。
详情请咨询Vectorlabs全国总代理商-欣博盛生物科技
代理品牌网站: www.nbs-bio.com
欣博盛品牌网站: www.neobioscience.net
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".. USA), and mouse anti-tartrate resistant acid phosphatase (TRAP) antibody (sc376875; Santa Cruz Biotechnology). ... To amplify the signal, we used biotinylated horse anti-mouse/rabbit IgG antibody (BA-1400; Vector Laboratories, Newark, CA, USA) and streptavidin–horseradish peroxidase (SA-5004; Vector Laboratories).. Immunoperoxidase staining was carried out using 3,3′-Diaminobenzidine tetrahydrochloride (DAB) (SK-4100, ImmPACT DAB Substrate kit, .." More...
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"The sections were subsequently treated with a biotinylated anti-mouse/rabbit IgG secondary antibody generated in horse (BA-1400, Vector Laboratories) for 2 h, followed by incubation with streptavidin conjugated to horseradish peroxidase (HRP) (SA-5004, Vector Laboratories ® ) for 1 h. For visualization, the reaction was developed using 3,3′-diaminobenzidine tetrahydrochloride (SK-4105, Vector Laboratories ® ) as the chromogen." More...
抗体结合，后者再与生物素标记的第二抗体结合，然后加入ABC复合物，结合到第二抗体的生物素上，最终形成的复合物网络了大量的酶分子。因此，敏感性更高。目前常用的亲和素从链霉菌(streptomyces)培养物中提取，因此称为链霉亲和素―生物素―过氧化物酶复合物技术(streptavidin biotin-peroxidase complex method，SABC法)。2．免疫组织化学酶类标记物 作为免疫酶组织化学标记物的酶以共价键的形式结合在抗体上，制成酶标抗体(SABC法是链霉亲和素―生物素与酶结合)，再借助酶
: Indirect immunohistochemistry with avidin-biotin peroxidase(e.g rabbit anti antigen X, goat anti-rabbit biotin, avidin HRP) 1- Wash twice in TBS 0.05M pH7.5, to which 0.01% Tween 20 has been added. 2- Briefly blot the slides without letting
Immunohistochemistry Protocol-Cytokine Antibodies
reveal the resulting peroxidase activity by incubating the slides with a 0.5 mg/mL HRP substrate solution (DAB + H2O2 prepared in distilled water). 11. Counterstain for 1 minute with hematoxylin. 12. Dehydrate slides with sequential ethanol
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