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文献和实验*发表【中文论文】请标注：由上海酶研生物科技有限公司提供；
*发表【英文论文】请标注：From Shanghai EK-Bioscience Biotechnology Co., Ltd.
Non-fluorescent protein/RNA double-labeling
% hydrogen peroxide in PBTH. This will produce a brown signal. Stop the reaction with several rinses of PBTH. In situ HYBRIDIZATION : 1. Fix the embryos for 20 minutes with 5% formaldehyde in PBT (PBT= filtered 1 X PBS with 0.1% Tween-20
Non-fluorescent protein/RNA double-labeling
of PBTH. In situ HYBRIDIZATION : 1. Fix the embryos for 20 minutes with 5% formaldehyde in PBT (PBT= filtered 1 X PBS with 0.1% Tween-20) at room temperature. 2. Wash 3 X 5 minutes with PBT. 3. Incubate the embryos with 50 ug/ml
RNA in situ protocol for DNA and RNA probes
tRNA, 100 ug/ml sonicated, boiled salmon sperm DNA, 0.1% Tween-20) and the duration of the hybridization buffer washes. This dramatically reduces the background, because the hybridization buffer is approximately 20oC more stringent than the PBT used
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