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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Histone H3R2me2s (symmetric) antibody (pAb), sample
- 供应商：
上海西宝
- 规格：
10 µl
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文献和实验to include a "no antibody" control sample. Adjust the final volume of each sample with lysis buffer if required. Sample volumes should be between 400-800µl/sample to allow ample mixing during the overnight incubation. Add 1µg of antibody to each sample
Chromatin Immunoprecipitation (ChIP)
should be ~1.4-1.6. 12. Aliquot 100 µg of chromatin per antibody to be used into microcentrifuge tubes. It is important to have an irrelevant antibody such as -glutathione S-transferase as a control. 13. Dilute the chromatin to a final
-5436. Noma, K.-I., Allis, C.D., and Grewal, S.I.S. 2001. Transitions in distinct histone H3 methylation patterns at the heterochromatin domain boundaries. Science 293: 1150-1155. Uejima, H., Lee, M.P., Cui, H., and Feinberg, A.P. 2000. Hot-stop PCR
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