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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海西宝
- 英文名:
Phospho-Histone H3 (Thr11) Polyclonal Antibody (25 ug)
- 规格:
25 ug
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文献和实验The optimal exposure time will depend on the amount of 32 P used and the amount of kinase activity. Generally a few hours should be fine. SOLUTIONS Histone Wash Buffer 25 mM Tris HCl, pH7.5 70 mM NaCl 10 mM MgCl2 1 mM DTT
k to remove the bound fractions and retain the unbound as our elute for the antibody pulldown. Though clearly we could reduce the spin. In the bead recover in section (11) we have added the sepharose beads at step (11) and then wash accordingly
Use of Polyamine Derivatives as Selective Histone Deacetylase Inhibitors
Histone acetylation and deacetylation, mediated by histone acetyltransferase and the 11 isoforms of histone deacetylase, play an important role in gene expression. Histone deacetylase inhibitors have found utility in the treatment of cancer
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