- ￥160
- 博尔森/BIOESN
- 中国/上海
- BES-20141CCM
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C避光保存
- 保质期：
见详情说明
- 库存：
20
- 供应商：
上海博尔森生物
- 规格：
500 ml
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文献和实验Procedure for Transfection of Mammalian Cells
IMDM containing 20% fetal bovine serum, 1% glutamine, 1% aa In a six-well or 35 mm tissue culture plate, seed ~2x 105 cells per well in 2 ml IMDM containing 10% FBS and nonessential amino acids. Incubate the cells at 37E C
(1)In a six-well or 35 mm tissue culture plate, seed ~2x 105 cells per well in 2 ml IMDM containing 10% FBS and nonessential amino acids. (2)Incubate the cells at 37E C in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare
Harvesting Hematopoietic Cells from Mice
Materials 4 mice from each genotype 4 Ly5 mice Buckets with wet ice 3x Bucket with dry ice 1x Dewar flask with liquid nitrogen 100 mL beakers with 95% ethanol 2x 100 mL beaker with dd. H2 O 2x
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