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文献和实验DIG (Digoxigenin) labeled RNA probe In situ hybridization protocol
Denhardt’s solution 5x 100 µl Dextran sulphate 10% 200 µl Heparin
Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
reconstitution of endosome docking/fusion and sorting/budding, which is based on labeling of endosomes by endocytotic uptake with fluorescent cargoes. The protocol includes (i) the preparation of fluorescently labeled endosomes, (ii) assays for docking/fusion
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