Polystyrene particles, strepta

Enzyme Immobilization on Polyethyleneimine-Coated Magnetite Particles

The use of enzymes in industrial or medical applications has been limited (except for proteases and some amylases) because most enzymes are relatively unstable and the cost of their isolation and purification is still high. In addition

Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads

during the amplification reaction. The DNA can then be immobilized onto streptavidin-coated paramagnetic beads, simultaneously removing buffers, dNTPs, and unincorporated PCR primers. By alkali denaturation, the immobilized double-stranded DNA is converted into a single

Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab)2, and Streptavidin

Colloidal gold probes, including protein A-, IgG-F(ab’ )2 -, and streptavidin-labeled gold particles, are useful tools for localization of antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different