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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
9999
- 保质期:
08/2024
- 供应商:
Celligent
- 保存条件:
-20°C
- 规格:
100 ml
Fetal bovine serum contains naturally huge amount of exosomes, carrying the potential risk to interfere with the exosomes secreted by the cells - a highly critical problem in exosome research. Our newly developed method to get rid of exosomes is efficient but gentle to other FBS components. In comparison with the non depleted FBS the cell culture performance is still outstanding.
Features:
- No time-consuming self-production needed anymore
- Reduction of the risk of contamination
- Each bottle with comparable quality
- Each lot tested for exosome depletion
- No impairment of important FBS ingredients
Product Specifications
| Appearance | Clear amber liquid |
| Storage and shelf life | Store at ≤ -15°C. |
| Avoid repeated freeze-thaw cycles. Preparation of aliquots recommended. | |
| Once opened, store at +4°C and use within 4-6 weeks. | |
| Shipping conditions | Frozen (Dry ice) |
| Thawing | Overnight at +2°C to +8°C. Swirl gently to homogenize. |
Quality Control
Only serum batches which pass our strict quality control are released for sale. Standard parameters which are determined include pH, osmolality, content of protein, albumin, IgG and hemoglobin, endotoxin level, sterility, mycoplasma detection and virus testing. The quality control for exosome-depleted FBS additionally includes exosome/Cd63 testing by an ELISA assay.
Precautions and Disclaimer
This product is for research use only.
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文献和实验)。仔细收集上清。检测细胞内的成份时,用 PBS ( PH7.2-7.4 )稀释细胞悬液,细胞浓度达到 100 万 /ml 左右。通过反复冻融,以使细胞破坏并放出细胞内成份。离心 20 分钟左右( 2000-3000 转 / 分)。仔细收集上清。保存过程中如有沉淀形成,应再次离心。 5. 组织标本:切割标本后,称取重量。加入一定量的 PBS , PH7.4 。用液氮迅速冷冻保存备用。标本融化后仍然保持 2-8℃ 的温度。加入一定量的 PBS ( PH7.4 ),用手工或匀浆器将标本匀浆
科研君们,当埋头实验几月找到了目标基因片段,电泳时条带却突然消失了;千辛万苦分离纯化的物质,色谱图中却意外出现杂质峰,苦苦查询,可能仍找不到问题原因。若问题只在小小的吸头上,科研君的心还能淡定吗? 吸头的问题在哪里,如何保证纯净无污染的吸头?需要考虑以下几方面因素: 一、 吸头材质 吸头市场上,原材料基本都是聚丙烯塑料(低吸附,化学惰性高,使用温度范围广,无色透明)。也许大家不知道的是,同样是聚丙烯,品质会有大的差别。高品质的吸头一般采用天然 100% 纯聚丙烯,而低廉的吸头则很多
Zymolyase Dissolve in 10% glucose. Store aliquots (25 µl) at 20o C in common freezer for stock solutions Use once and dispose Comments: alternative is to dissolve in SCE, 40% gycerol, 20 mg/ml final. Can use
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