- ￥2460
- AAT Bioquest
- AAT-5301
- 进口
- 2025年07月15日
7 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Phalloidin *CAS#: 17466-45-4*
- CAS号：
17466-45-4
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
1 mg
齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电
关键词：鬼笔环肽Phalloidin CAS 17466-45-4详细如下
Phalloidin, a bicyclic heptapeptide toxin, binds specifically at the interface between F-actin subunits, locking adjacent subunits together. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Thus, phalloidin traps actin monomers in a conformation distinct from G-actin and it stabilizes the structure of F-actin by greatly reducing the rate constant for monomer dissociation, an event associated with the trapping of ADP. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
关键词：鬼笔环肽Phalloidin CAS 17466-45-4详细如下
Phalloidin, a bicyclic heptapeptide toxin, binds specifically at the interface between F-actin subunits, locking adjacent subunits together. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Thus, phalloidin traps actin monomers in a conformation distinct from G-actin and it stabilizes the structure of F-actin by greatly reducing the rate constant for monomer dissociation, an event associated with the trapping of ADP. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
| 货号 | 英文名称 | 规格 |
| AAT-104300Y0 | mFluor™ UV460 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-104300Y1 | mFluor™ UV460 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-104300Z0 | mFluor™ Violet 450 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-104300Z1 | mFluor™ Violet 450 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430100 | mFluor™ Violet 500 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430101 | mFluor™ Violet 500 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430110 | mFluor™ Violet 510 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430111 | mFluor™ Violet 510 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430120 | mFluor™ Violet 540 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430121 | mFluor™ Violet 540 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430140 | AF350 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430141 | AF350 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430150 | AF488 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430151 | AF488 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430160 | AF555 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430161 | AF555 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430170 | AF594 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430171 | AF594 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430180 | AF647 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430181 | AF647 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-10430190 | AF680 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-10430191 | AF680 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-104301A0 | AF700 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-104301A1 | AF700 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-104301B0 | AF750 Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-104301B1 | AF750 Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-104301C0 | APC Anti-human CD43 Antibody *HI165* | 25tests |
| AAT-104301C1 | APC Anti-human CD43 Antibody *HI165* | 100tests |
| AAT-104301C2 | APC Anti-human CD43 Antibody *HI165* | 500tests |
| AAT-104301D0 | APC/Cy7 Anti-human CD43 Antibody *HI165* | 25tests |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
最近，随着基因编辑技术不断曝光，这项技术也越来越火，越来越受到关注，但大部分媒体都只是浅尝辄止进行宣传而已，实质性的怎么操作，具体的步骤却很少涉及。鉴于此，师兄就把张峰的手稿扒拉出来，分享给大家。同时也欢迎大家与我探讨，我的微信号;shixiongcoming，向我申请的时候注意，写上你的理由哦，最近加我的人太多，不注明来由的读者，别怪师兄拒绝你呦。 Genome Engineering Using CRISPR-Cas9 System Le Cong
CRISPR/Cas9 系统作为细菌和古细菌的获得性免疫系统， 通过 RNA 介导特异性的切割外源遗传物质， 用以对抗入侵的病毒和质粒。利用 Cas9 来摧毁入侵 DNA 的 Type Ⅱ CRISPR/Cas 系统， 可以在体外进行基因的编辑。为了提高CRISPR/Cas9 的特异性，使用 Cas9 切口酶和一对 sgRNA，两个相近的切口造成 DNA 双链断裂， 诱导细胞发生非同源末端连接修复， 造成目的基因的突变。利用 CRISPR/Cas9 进行基因的编辑，首先要构建有效的 sgRNA
一、试剂准备 1、Cas9 过表达慢病毒载体选择。 货号 名称 CR2001 pLV-Cas9-Puro CR2002 pLV-Cas9-Neo CR2003 pLV-Cas9Nick-Puro CR2004
技术资料暂无技术资料 索取技术资料
鬼笔环肽Phalloidin CAS 17466-45-4
￥2460
