- ￥2200
- Biorigin
- BN40893R
- 2025年08月02日
- WB,
- Human,Mouse,Rat,Dog,
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
Human,Mouse,Rat,Dog,
- 应用范围：
WB,
- 抗体英文名：
Phospho-Mre11 (Ser676)
- 规格：
100ul
| 英文名称 | Phospho-Mre11 (Ser676) |
| 中文名称 | 磷酸化DNA损伤关键蛋白Mre11抗体 |
| 别 名 | Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 meiotic recombination 11 homolog A; MRE11a; MRE11b; AT like disease; Ataxia telangiectasia disorder like; Ataxia-telangiectasia disorder-like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11. HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; meiotic recombination 11 homolog A (S. cerevisiae); meiotic recombination 11 homolog A; MmMRE11A.MRE11_RAT |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 免疫学 染色质和核信号 细胞凋亡 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Mouse, Rat, Dog, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 80kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from rat Mre11 around the phosphorylation site of Ser676:SQ(p-S)Q |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Function: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation Subunit: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Subcellular Location: Nucleus. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. DISEASE: Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. Similarity: Belongs to the MRE11/RAD32 family. SWISS: P49959 Gene ID: 64046 Database links: Entrez Gene: 4361 Human Entrez Gene: 17535 Mouse Omim: 600814 Human SwissProt: P49959 Human SwissProt: Q61216 Mouse Unigene: 192649 Human Unigene: 149071 Mouse Unigene: 209040 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 在细胞中，有多种蛋白参与DNA损伤应答，DNA损伤是可引起癌变的细胞变化，这些蛋白在细胞发生损伤后会启动细胞修复过程，帮助受损的细胞恢复正常。 正常情况下，细胞会经历生长、分化和自然死亡的历程。当细胞受到损伤时，如;辐射损伤或是毒物刺激，一种多蛋白复合物参与的步骤将被启动，进行细胞修复工作并激活其他的生物过程。在这过程中，存在一种MRN复合物，由Mre11，Rad50和NBS1蛋白组成，MRN探测DNA损伤（DNA双链是否断裂）的情况。复合物在探测到DNA损伤信号后将把这个信息传递给一种酶，ATM（ataxia-telangiectasia mutated）检测激酶（checkpoint kinase）。ATM激酶能对DNA双链断裂产生应答反应，它具有降低细胞增殖的能力，给细胞修复腾出时间。因此ATM一旦发生变异，功能失效可能导致免疫缺陷甚至是癌变。 研究者认为，Mre11不仅是DNA损伤的感受器，更是修复DNA的启动因子，还能修饰受损的DNA分子 |
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验上聚集 利用 405 nm 激光对表达 GFP-MRE11 的 U2OS 细胞进行激光诱导 DNA 损伤，然后用 488 nm 激光对 GFP 进行时间序列成像。以 20 秒间隔对细胞成像 6 分钟，以便对 DNA 损伤前后 MRE11 的定位进行可视化和量化。 超级色差校正物镜实现精确的共定位分析 除了研究 MRE11 募集至链断裂位点的动力学过程之外，我们还检查了γH2AX（γH2AX 是一种在 DNA 双链断裂处被磷酸化并激活 DDR 通路的组蛋白）和 CHD4（一种在表观遗传转录
Mol Cell：杨薇等揭示 DNA-PK 的激活机制以及自磷酸化对 NHEJ 通路的重要作用
DNA 双链断裂可由外在因素（电离辐射和活性氧等）或内在因素（DNA 复制错误和 V（D）J 重组等）造成，从而激发 DNA 损伤应答，包括 DNA 修复、细胞周期调控、细胞衰老和细胞凋亡等。 DNA 损伤应答由 PIKK（phosphoinositide-3-kinase-related kinase）家族蛋白激酶催化下游蛋白的磷酸化而启动，包括 DNA-PK（DNA 依赖性蛋白激酶）、ATM（Ataxia Telangiectasia Mutated）和 ATR（ATM-Related
浙江大学柯越海课题组发文解析去磷酸化修饰调控细胞外囊泡的作用机制
基础，也是药物干预的关键靶点，其中酪氨酸磷酸化是广受关注的新药靶点。 该研究首先发现一种非受体型酪氨酸磷酸酶 PTPN11/Shp2 缺失或者抑制会显著增加多种细胞的外泌体分泌，通过筛选并鉴定了磷酸酶 PTPN11/Shp2 可直接修饰外泌体生成的关键蛋白 Syntenin 第 46 位酪氨酸的去磷酸化，进而影响 Syntenin-Alix 胞内复合物，控制囊泡转运的内体分选复合体（ESCRT）形成。 该研究首次发现去磷酸化修饰是胞外囊泡的合成与分泌的一个潜在的控制因素，有多种酪氨酸磷酸酶参与
技术资料暂无技术资料 索取技术资料
