- ￥1900
- Biorigin
- BN40783R
- 2025年08月02日
- WB,
- Human,Mouse,Rat,
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- 详细信息
- 文献和实验
- 技术资料
- 适应物种：
Human,Mouse,Rat,
- 应用范围：
WB,
- 抗体英文名：
IRS-4
- 规格：
100ul
| 英文名称 | IRS-4 |
| 中文名称 | 胰岛素受体底物-4抗体 |
| 别 名 | IRS4; 160 kDa phosphotyrosine protein; Insulin receptor substrate 4; IRS 4; PY160; IRS4_HUMAN; IRS-4; Phosphoprotein of 160 kDa; pp160. |
| 研究领域 | 心血管 信号转导 细胞凋亡 糖尿病 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 134kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IRS-4:551-650/1257 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| PubMed | PubMed |
| 产品介绍 | IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation. Function: Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May acts as negative regulators of the IGFI signaling pathway by suppressing the function of IRS1 and IRS2. Subunit: Interacts with CRK and CRKL. Interaction with CRK is stronger than with CRKL. Interacts with CRK via the phosphorylated YXXM motifs. Interacts with PLC-gamma, SHC1, PTK6, PPP4C and NISCH. Interacts with SOCS6 in response to stimulatiom with either insulin or IGF1. Interacts with PIK3R1 and GRB2. Subcellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Tissue Specificity: Expressed in skeletal muscle, brain, heart, kidney and liver. Post-translational modifications: Phosphorylated on tyrosine residues in response to both insulin and IGF1 signaling. Phosphorylated on Tyr-894 in response to FGF2 signaling. Phosphorylation of Tyr-894 is required for GRB2, phospholipase C-gamma and phosphatidylinositol 3-kinase interaction. Similarity: Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: O14654 Gene ID: 8471 Database links: Entrez Gene: 8471 Human Entrez Gene: 16370 Mouse Omim: 603510 Human SwissProt: O14654 Human SwissProt: Q9Z0Y7 Mouse Unigene: 460872 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验胰岛素受体底物(insulin receptor substrate，IRSs)
能够被激活的胰岛素受体酪氨酸激酶作用的底物, 其上具有十几个酪氨酸残基可被磷酸化，磷酸化的IRSs能够结合并激活下游效应物。 IRSs在被胰岛素受体磷酸化以后，如同一块“磁铁”与那些具有SH2结构域的蛋白结合，根据所结合蛋白的具体结构产生不同的效应，如激活SH2蛋白的酶活性、改变蛋白质构型并同另外的蛋白结合或者引起蛋白质从细胞的一个部位转移到另一个部位。 已知有三种胰岛素受体酪氨酸激酶作用的底物(IRSs)。第一种是胰岛素受体底物1(IRS1),是一种
复合物，经过彻底洗涤后加底物 TMB 显色。 TMB 在 HRP 酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗胰岛素自体抗体 (IAA) 呈正相关。用酶标仪在 450nm 波长下测定吸光度（ OD 值），通过标准曲线计算样品中人抗胰岛素自体抗体 (IAA) 浓度。 试剂盒组成 ： 试剂盒组成 48 孔配置
胰岛素受体是一个四聚体，由两个α亚基和两个β亚基通过二硫键连接。两个α亚基位于细胞质膜的外侧，其上有胰岛素的结合位点；两个β亚基是跨膜蛋白，起信号转导作用。无胰岛素结合时，受体的酪氨酸蛋白激酶没有活性。当胰岛素与受体的α亚基结合并改变了β亚基的构型后，酪氨酸蛋白激酶才被激活，激活后可催化两个反应∶①使四聚体复合物中β亚基特异位点的酪氨酸残基磷酸化,这种过程称为自我磷酸化(autophosphorylation)；②将胰岛素受体底物(insulin receptor substrate
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