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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
OVCAR8
- 库存:
1x10^6/瓶
- 供应商:
上海酶研
- 肿瘤类型:
卵巢癌
- 细胞类型:
OVCAR8
- 品系:
OVCAR8
- 组织来源:
人卵巢癌细胞
- 相关疾病:
详询
- 物种来源:
人
- 免疫类型:
详询
- 细胞形态:
贴壁/悬浮
- 是否是肿瘤细胞:
是
- 器官来源:
人卵巢癌细胞
- 运输方式:
顺丰快递
- 年限:
5年
- 生长状态:
生长良好
OVCAR-8细胞系 、OVCAR8细胞株 、OVCAR-8 细胞、OVCAR8 细胞、OVCAR-8 卵巢癌细胞
Cell line name OVCAR-8
Synonyms OVCAR 8; NIH:OVCAR-8; OVCAR8; Ovcar8; OVCAR.8; OVCA8
Accession CVCL_1629
Resource Identification Initiative To cite this cell line use: OVCAR-8 (RRID:CVCL_1629)
Comments Part of: Cancer Dependency Map project (DepMap) (includes Cancer Cell Line Encyclopedia - CCLE).
Part of: COSMIC cell lines project.
Part of: JFCR39 cancer cell line panel.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
Part of: NCI-60 cancer cell line panel.
Population: Caucasian.
Doubling time: 24 hours (PubMed=7718330); 25 hours (PubMed=25984343); 26.1 hours (NCI-DTP=OVCAR-8); 31.93 hours (JWGray panel).
Microsatellite instability: Stable (MSS) (Sanger).
Omics: Array-based CGH.
Omics: Chromatin accessibility by ATAC-seq.
Omics: CNV analysis.
Omics: CRISPR phenotypic screen.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: DNA methylation analysis.
Omics: Fluorescence phenotype profiling.
Omics: Genome sequenced.
Omics: lncRNA expression profiling.
Omics: Metabolome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: shRNA library screening.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Omics: Transcriptome analysis by RNAseq.
Misspelling: OVACAR8; Cosmic=1526923.
Misspelling: OVOCAR-8; Note=Occasionally.
Misspelling: OVOCAR8; Note=Occasionally.
PubMed=10700174; DOI=10.1038/73432
Ross D.T., Scherf U., Eisen M.B., Perou C.M., Rees C., Spellman P.T., Iyer V.R., Jeffrey S.S., van de Rijn M., Waltham M.C., Pergamenschikov A., Lee J.C.F., Lashkari D., Shalon D., Myers T.G., Weinstein J.N., Botstein D., Brown P.O.
Systematic variation in gene expression patterns in human cancer cell lines.
Nat. Genet. 24:227-235(2000)
PubMed=12417041; DOI=10.1111/j.1349-7006.2002.tb01213.x; PMCID=PMC5926887
Watanabe T., Imoto I., Katahira T., Hirasawa A., Ishiwata I., Emi M., Takayama M., Sato A., Inazawa J.
Differentially regulated genes as putative targets of amplifications at 20q in ovarian cancers.
Jpn. J. Cancer Res. 93:1114-1122(2002)
PubMed=12960427; DOI=10.1091/mbc.E03-05-0279; PMCID=PMC266758
Schaner M.E., Ross D.T., Ciaravino G., Sorlie T., Troyanskaya O.G., Diehn M., Wang Y.C., Duran G.E., Sikic T.L., Caldeira S., Skomedal H., Tu I.-P., Hernandez-Boussard T., Johnson S.W., O'Dwyer P.J., Fero M.J., Kristensen G.B., Borresen-Dale A.-L., Hastie T., Tibshirani R., van de Rijn M., Teng N.N.H., Longacre T.A., Botstein D., Brown P.O., Sikic B.I.
Gene expression patterns in ovarian carcinomas.
Mol. Biol. Cell 14:4376-4386(2003)
PubMed=15748285; DOI=10.1186/1479-5876-3-11; PMCID=PMC555742
Adams S., Robbins F.-M., Chen D., Wagage D., Holbeck S.L., Morse H.C. 3rd, Stroncek D., Marincola F.M.
HLA class I and II genotype of the NCI-60 cell lines.
J. Transl. Med. 3:11.1-11.8(2005)
PubMed=17088437; DOI=10.1158/1535-7163.MCT-06-0433; PMCID=PMC2705832
Ikediobi O.N., Davies H.R., Bignell G.R., Edkins S., Stevens C., O'Meara S., Santarius T., Avis T., Barthorpe S., Brackenbury L., Buck G., Butler A.P., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Hunter C., Jenkinson A., Jones D., Kosmidou V., Lugg R., Menzies A., MiroT., Parker A., Perry J., Raine K.M., Richardson D., Shepherd R., Small A., Smith R., Solomon H., Stephens P.J., Teague J.W., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Reinhold W.C., Weinstein J.N., Stratton M.R., Futreal P.A., Wooster R.
Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
Mol. Cancer Ther. 5:2606-2612(2006)
PubMed=19372543; DOI=10.1158/1535-7163.MCT-08-0921; PMCID=PMC4020356
Lorenzi P.L., Reinhold W.C., Varma S., Hutchinson A.A., Pommier Y., Chanock S.J., Weinstein J.N.
DNA fingerprinting of the NCI-60 cell line panel.
Mol. Cancer Ther. 8:713-724(2009)
PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Bignell G.R., Greenman C.D., Davies H.R., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)
PubMed=20204287; DOI=10.3892/or_00000728; PMCID=PMC2909445
Langland G.T., Yannone S.M., Langland R.A., Nakao A., Guan Y.-H., Long S.B.T., Vonguyen L., Chen D.J., Gray J.W., Chen F.-Q.
Radiosensitivity profiles from a panel of ovarian cancer cell lines exhibiting genetic alterations in p53 and disparate DNA-dependent protein kinase activities.
Oncol. Rep. 23:1021-1026(2010)
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文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
qijianniheihei 肿瘤细胞系和自然生长的肿瘤是有差异的。在肿瘤细胞系表达的基因不一定在自然生长的肿瘤中有表达;相反,同上。 linghe82 那要研究这种蛋白在卵巢癌中的作用,就需要将这种蛋白转染到癌细胞中进行表达 dnazyme freecell wrote: “在肿瘤里有表达”是什么概念?是怎样检测到的? 这个提问已经点到点子上了。
我所用的卵巢癌的细胞包括SKOV3、A2780、3AO、OVCAR3、HRA,几种细胞的特性不完全一样,但是我的传代的步骤是基本一致的:细胞生长至80%汇合时传代,先倒掉培养液,加预先加好双抗的生理盐水或PBS 5-10ml(100ml培养瓶)后轻轻摇晃数次后倒掉,加0.25-0.35%的胰酶2ml,并使其分布均匀(这一点很重要,一定要注意工作台是否平整,否则容易造成胰酶分布不均匀而细胞消化不同步),待细胞间隙变大时终止消化,加含10%血清的培养液(我用的是1640)4ml轻柔吹打,再按照需
相关专题 各种已被命名和经过细胞生物学鉴定的细胞系或细胞株,都是一些形态比较均一、生长增殖比较稳定的和生物性状清楚的细胞群。因此凡符合上述情况的细胞群也可给以相应的名称,即文献中常称之为已鉴定的细胞(Certified Cell s)。已鉴定的细胞可用于各种实验研究和生产生物制品。当前世界上已建的各种细胞系(株)已难胜数,我国也建有百种以上,并在不断增长中。 一、体外培养细胞的种类和命名 体外培养细胞的名称
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