- ￥2700
- origene
- TA500654
- 2025年07月06日
- WB 1:200 - 1:1000, IHC 1:50, IF 1:100, IP 2ug/500ul
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
WB 1:200 - 1:1000, IHC 1:50, IF 1:100, IP 2ug/500ul
- 适应物种：
Human
- 规格：
100ul
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文献和实验Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
(1)Cell Fusion and Selection Solutions: - IMDM - IMDM complete + 15% MCM. - PBS - PEG 50% ( w/v ): PEG 4000 ( 50% ) from Boehringer Manheim 1 243 268 - Aminopterin: Sigma, A 5159 (50X ) Prepare a T-150 flask with 170ml of IMDM
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