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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
StrandBrite™ Green RNA Quantifying Reagent *200X DMSO Solution*
- CAS号：
/
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
1 ml
关键词：StrandBrite 绿色RNA定量试剂 200X DMSO溶解详细如下
Detecting and quantitating small amounts of RNA is extremely important for a wide variety of molecular biology procedures such as measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analysis, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR. The most commonly used technique for measuring nucleic acid concentration is the determination of absorbance at 260 nm. The major disadvantage of the absorbance-based method is the interferences caused by proteins, free nucleotides and other UV absorbing compounds. The use of sensitive, fluorescent nucleic acid stains alleviates this interference problem. StrandBrite™ RNA quantifying reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. It is a positively charged fluorescent probe that binds to the hydrophobic pockets of RNA and forms a highly luminescent complex through the synergistic actions of stacking, hydrophobic forces, hydrogen bonding and electrostatic interactions. StrandBrite™ RNA quantifying reagent has extremely low inherent fluorescence that is significantly enhanced upon binding to RNAs, resulting in a great enhancement in its fluorescence. StrandBrite™ RNA quantifying reagent can detect as little as 5 ng/mL RNA with a fluorescence microplate reader or fluorometer.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下：
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文献和实验个细胞。考虑到我们后续还要对酶切用量进行优化，实际准备了足量细胞（每组约 1.5×107 个细胞）。在我们实验前，我们仔细观察了细胞的状态和密度，细胞状态很健康，贴壁良好，也达到了 80% 左右的汇合率，一切都符合 ChIP 实验的要求。拿出试剂盒，我们首先对各种标配试剂置于冰浴中进行了标记。由于试剂盒标配的 DTT 为干粉，因此我们需要配制 1M DTT 溶液（具体地，192.8 mg DTT#7016 加 1.12 ml dH2O，确保 DTT 完全溶解）。然后，我们取出并室温预热 200X
input量少；可结合Quantseq 3’mRNA建库试剂盒使用，节省大量的测序空间，大大节约测序成本，使其成为RNA代谢动力学研究的首选，同时，SLAM-seq技术方法学发表在了Nature Methods上。 图3 SLAMseq工作流示意图。培养的细胞用4-硫代尿苷(S4U)标记新生RNA(绿色)。抽提纯化总RNA，并加入碘乙酰胺(IAA)诱导4-巯基烷基化。用QuantSeq 3’ mRNA-Seq文库构建试剂盒进行文库构建，在反转录过程中，S4U位点会反转录成鸟嘌呤 (G，红色
没什么差别，而且染色结果非红即绿，这是怎么回事？ sunandsuny: 正常的细胞核的DNA应该呈现比较均匀的黄色或黄绿色的荧光,而RNA呈现黄色或橘红色,,在凋亡时,细胞核和细胞质内呈现致密浓染的黄绿色荧光,甚至是黄绿色碎片.而当细胞坏死时,核溶解,减少，上述黄绿色荧光减少或消失. 所以楼上所说的"非红即绿"可能就出现了凋亡现象,你最好上传一长照片看看. 还有,最好用活细胞染色较好,不要固定.激发光 playboyzmj: 看到大家用AO-EB的方法来检测细胞凋亡。但有几个问题一直搞不懂
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