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- Invitrogen™
- R11490
- 2025年07月04日
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- 英文名：
Quant-iT™ RiboGreen RNA 定量试剂盒与 RiboGreen RNA 试剂、RediPlate™ 96 RiboGreen™ RNA 定量试剂盒
- CAS号：
见产品外包装
- 保质期：
见产品外包装
- 供应商：
嵘崴达
- 保存条件：
见产品外包装
- 规格：
1 kit
Quant-iT™ RiboGreen RNA 定量试剂盒与 RiboGreen RNA 试剂、RediPlate™ 96 RiboGreen™ RNA 定量试剂盒
Workflow
This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection. To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL. RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.
Applications
Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.
上海嵘崴达实业有限公司，专业经营生命科学领域的科研试剂、仪器和实验室消耗品，
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主要经营的试剂品牌：
Roche试剂，部分常用现货，周三定货，2-3周到货。
GE试剂，耗材，部分常用现货，周二，周五定货，3周左右。
SIGMA试剂，周一，五定货，3周左右到货。
THERMO试剂，部分现货，周三定货，2-3周到货。
ABCAM试剂，国内现货三天左右，期货四周左右。
Peprotech试剂，部分常用现货，周三定货，2-3周到货。
罗氏原料，请来电来邮咨询。
OXOID，BD培养基货期八周左右，部分现货。
MCE激动剂，ACROS、ALFA、阿拉丁等生化试剂现货定购。
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文献和实验使用Lipofectamine™2000转染Stealth™ RNA或者siRNA进入哺乳动物细胞
Lipofectamine™ 2000试剂（使用前贮存在+4℃） ・Opti-MEM® I 低血清培养基（使用前37℃预热） ・合适的组织培养板及其它 转染步骤 使用Lipofectamine™ 2000转染Stealth™ RNA或者siRNA进入哺乳动物细胞时，使用下述步骤。参阅下列表格《推荐试剂的量和体积》以确定加入不同组织培养方式的试剂量以及体积。使用推荐Lipofectamine™ 2000的量
methods for accurately quantitating small amounts of RNA two are described below. Fluorescent Dyes Certain fluorescent dyes, such as RiboGreen® (Molecular Probes), exhibit a large fluorescence enhancement when bound to nucleic acids. As little as 1
, there are alternative methods for accurately quantitating small amounts of RNA two are described below. Fluorescent Dyes Certain fluorescent dyes, such as RiboGreen® (Molecular Probes), exhibit a large fluorescence enhancement when bound to nucleic acids
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