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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Hoechst 33258 *CAS 23491-45-4*
- CAS号：
23491-45-4
- 保质期：
详见说明
- 供应商：
齐源生物
- 保存条件：
详见说明
- 规格：
100 mg
关键词：细胞核染料 Hoechst 33258 CAS 23491-45-4详细如下
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant. These dyes can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.
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文献和实验Hoechst 33258染色1．原理 Hoechst 33258和Hoechst 33342均为非嵌人性荧光染料。它们在活细胞中 DNA聚AT序列富集区域的小沟处与DNA结合。活细胞或固定细胞均可从低浓度溶液中 摄取该染料。从而使细胞核着色。故又把此类染料称为DNA探针。Hoechst 33342和Hoechst 33258均可溶于水并在水溶液中保持稳定。Hoechsr-DNA的激发和发射波长分别550nm和460nm。在荧光显微镜紫外光激发时，Hoechst-DNA发出亮
：PH和氯化钠浓度对于Hoechst试剂和DNA的结合至关重要。 1倍的TNE:用90ml蒸馏水（0.45μm滤膜过滤）稀释10ml 10倍的TNE。 4.3 准配2倍的染料液用于10-1000 ng/ml的DNA：用100ml的1倍的TNE稀释20ul Hoechst 33258 储存液 (1 mg/ml)，室温放置，现用现配，一旦染料加入千万别再过滤！ 4.4 小牛胸腺DNA标准液：准备溶解
Hoechst33258（或Hoechst33342）染色法检测细胞凋亡
涂片或细胞甩片用0.1%多聚甲醛固定30min，用PBS洗5min； 将以上制备好的玻片，浸泡在装有Hoechst33258（或Hoechst33342）染液的染色缸中，避光作用10min； 封片。 【结果判断】 置于荧光显微镜下，选用340nm的激发光观察：在荧光显微镜下，活细胞呈弥散均匀荧光，凋亡细胞核或细胞质内可见浓染致密的颗粒块状荧光。如果见到3个或3个以上的DNA荧光碎片被认为是凋亡细胞。
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