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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Readiuse™ Rapid Luminometric ATP Assay Kit
- CAS号:
/
- 保质期:
详见说明
- 供应商:
齐源生物
- 保存条件:
详见说明
- 规格:
100 Tests
关键词:Readiuse 快速发光法ATP检测试剂盒详细如下
Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling. The quantitation of ATP can be used for a variety of biological applications. Because ATP is the energy source for almost all living organisms that rapidly degrades in the absence of viable organisms, its existence can be used to identify the presence of viable organisms. The measurement of ATP has been used for cell cytotoxicity, detection of bacteria on surfaces, quantification of bacteria in water, somatic cells in culture and food quality. The use of firefly bioluminescence to measure ATP was first proposed by McElroy when he discovered that ATP was essential for light production. Firefly luciferase is a monomeric 61 kD enzyme that catalyzes a two-step oxidation of luciferin, which yields light at 560 nm. The first step involves the activation of the protein by ATP to produce a reactive mixed anhydride intermediate. In the second step, the active intermediate reacts with oxygen to create a transient dioxetane, which quickly breaks down to the oxidized product oxyluciferin and carbon dioxide along with a burst of light. When ATP is the limiting component, the intensity of light is proportional to the concentration of ATP. Thus the measurement of the light intensity can be used for quantifying ATP using a luminometer. AAT Bioquest's ReadiUse™ Rapid Luminometric ATP Assay Kit (DTT free) comes with all the essential components in a ready-to-use format. It provides a fast, simple and homogeneous luminescence assay for monitoring cell proliferation and cytotoxicity in mammalian cells. This assay is based on the detection of ATP using firefly luciferase to catalyze the release of light by ATP and luciferin. It can be performed in a convenient 96-well or 384-well microtiter-plate format on a chemiluminescent microplate reader. The assay is extremely sensitive and can detect 50 cells/well. It has stable luminescent signal with half-life more than 2 hours. This ReadiUse™ Rapid Luminometric ATP Assay Kit does not use DTT, which eliminating the unpleasant odor.
齐源生物专业代理美国AAT Bioquest、其它产品详细如下:
| 货号 | 英文名称 | 规格 |
| AAT-10060070 | iFluor™ 532 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-10060071 | iFluor™ 532 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-10060080 | iFluor™ 546 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-10060081 | iFluor™ 546 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-10060090 | iFluor™ 555 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-10060091 | iFluor™ 555 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600A0 | iFluor™ 560 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600A1 | iFluor™ 560 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600B0 | iFluor™ 568 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600B1 | iFluor™ 568 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600C0 | iFluor™ 594 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600C1 | iFluor™ 594 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600D0 | iFluor™ 610 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600D1 | iFluor™ 610 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600E0 | iFluor™ 633 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600E1 | iFluor™ 633 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600F0 | iFluor™ 647 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600F1 | iFluor™ 647 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600G0 | iFluor™ 660 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600G1 | iFluor™ 660 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600H0 | iFluor™ 670 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600H1 | iFluor™ 670 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600I0 | iFluor™ 680 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600I1 | iFluor™ 680 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600J0 | iFluor™ 700 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600J1 | iFluor™ 700 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600K0 | iFluor™ 710 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600K1 | iFluor™ 710 Anti-human CD6 Antibody *HI210* | 500tests |
| AAT-100600L0 | iFluor™ 750 Anti-human CD6 Antibody *HI210* | 100tests |
| AAT-100600L1 | iFluor™ 750 Anti-human CD6 Antibody *HI210* | 500tests |
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文献和实验1月26日从兰州省科技厅了解到,兰州雅华生物技术有限公司研究人员经过4年科研攻关,在结核病检测器械方面取得新突破——研制成功了结核抗体检测试剂盒,为结核病确诊提供了快速可靠的检测手段。 结核病传染性较强,传播途径广,在短时间内可使接触者感染。及早确认结核感染对患者治疗尤其重要。我国是全球22个结核病高负担国家之一,感染结核分枝杆菌的人数超过4亿,对结核病的综合防治是我国面临的重要公共卫生问题。 为解决结核诊断确诊率,提高结核菌的检测灵敏度,兰州雅华
:分别是竞争性蛋白质结合分析法[3]、放射性免疫分析法[4]和薄层色谱法[5]。在80年代,放射性免疫分析法得到了广泛的应用和改进,由于这种方法采用放射性核素标记,应用范围受到一定限制,为了进一步提高检测的灵敏度和操作的安全性,进入90年代又陆续发明了各种酶标记法和化学发光法的竞争性ELISA检测试剂盒,这一类检测方法的根本原理都是基于抗体抗原的免疫反应,所有这些试剂盒中采用的抗体全部是兔抗血清或者是经过特异亲和提纯之后的抗cAMP或cGMP的兔多抗,毫无疑问,兔多抗在约四十年的cAMP,cGMP定量
还有就是用Invitrogen公司的CFSE细胞增殖流式检测试剂盒也可,我觉得。 qing0831 可以用流式细胞术检测,我经常做这方面的实验,可以交流一下,手机:15921609870 QQ:57325595 bigbang_0_0 cologenic assay bigbang_0_0 cologenic assay nmmxq Brdu incorporation assay
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