万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名:
BEL-7402
- 库存:
999
- 供应商:
上海酶研
- 肿瘤类型:
肝癌
- 细胞类型:
详询
- ATCC Number:
详询
- 品系:
详询
- 组织来源:
详询
- 相关疾病:
详询
- 物种来源:
哺乳动物
- 免疫类型:
详询
- 细胞形态:
正常
- 是否是肿瘤细胞:
是
- 器官来源:
详询
- 运输方式:
常温/干冰
- 年限:
详询
- 生长状态:
良好
- 规格:
T25
Cell line name BEL-7402
Synonyms Bel-7402; BEL 7402; Bel 7402; BEL7402; Bel7402
Accession CVCL_5492
Resource Identification Initiative To cite this cell line use: BEL-7402 (RRID:CVCL_5492)
Comments Problematic cell line: Contaminated. Shown to be a HeLa derivative (PubMed=28807831). Originally thought to originate from a 53 year old male patient with a hepatocellular carcinoma.
Registration: International Cell Line Authentication Committee, Register of Misidentified Cell Lines; ICLAC-00549.
Population: African American.
Doubling time: 20 hours (PubMed=6154311); 31.2 +- 1.1 hours (PubMed=23167424).
Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18).
Omics: Deep quantitative proteome analysis.
Omics: SNP array analysis.
Omics: Transcriptome analysis by microarray.
Miscellaneous: Formerly the CCRID database had 2 entries describing this cell line (3131C0001000700010, 3142C0001000000035). They were the sole source for the STR profile of this entry.
Derived from site: In situ; Uterus, cervix; UBERON=UBERON_0000002.
Disease Human papillomavirus-related cervical adenocarcinoma (NCIt: C27677)
Species of origin Homo sapiens (Human) (NCBI Taxonomy: 9606)
Hierarchy Parent: CVCL_0030 (HeLa)
Children:
CVCL_5493 (BEL-7402/5-FU) CVCL_M184 (BEL-7402/5'-DFUR) CVCL_D8ER (Ubigene BEL-7402 KLF14 KO)
Sex of cell Female
Age at sampling 30Y6M
Category Cancer cell line
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
- 作者
- 内容
- 询问日期
文献和实验*发表【中文论文】请标注:由上海酶研生物科技有限公司提供;
*发表【英文论文】请标注:From Shanghai EK-Bioscience Biotechnology Co., Ltd.
AbstractAIM:To study the effect of a varying concentrations of ars enic trioxide on human hepatoma cell line BEL-7402 cultured in vitro and its mechanism of action.METHODS:The BEL-7402 cells were treated with arsenic trioxide (at the concentrations
肝刺激因子对人肝癌细胞BEL-7402 p21ras表达的影响
吸光值, 并与蛋白浓度成正比。以BSA为标准品求得标准曲线, 进而计算未知蛋白样品的浓度。 1.4 HSS活性的体外测定 实验采用MTT法[20]判定细胞增殖状况。HSS粗提物经Sephadex G-75层析后, 根据其OD280可绘成3个蛋白峰。选用人肝癌细胞株BEL-7402, 以含10%胎牛血清(美国HyClone公司)的DMEM(美国GIBCO/BRL公司)培养, 按5×104/ml细胞密度接种于96孔培养板, 每孔含5×103个细胞, 于37℃、5% CO2培养箱中培养。待细胞完全贴壁
实验动物、噬菌体肽库和细胞株 实验用荷瘤裸鼠购自上海实验动物中心,噬菌体12肽文库及大肠杆菌ER2738购自美国NewEnglandBiolab公司,人源肝癌细胞BEL―7402、正常肝细胞HL―7702购自中国科学院上海生物化学及细胞生物学研究所细胞库。 2.试剂 RPMI―1640培养基购自美国GIBCO公司,小牛血清购自杭州四季青公司;PEG―8000购自美国Amresco公司,兔抗M13噬菌体血清由澳大利亚CSIRO动物健康研究
技术资料






