Amplite 比色法L-乳酸检测试剂盒

齐源生物专业代理美国AAT Bioquest产品，并有大量储存现货欢迎致电
关键词：Amplite 比色法L-乳酸检测试剂盒详细如下
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid. Lactate is constantly produced from pyruvate via the enzyme lactate dehydrogenase (LDH) in the process of metabolism and exercise. Monitoring lactate levels is a good way to evaluate the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is not metabolized by mammals and its elimination from the body depends mainly on renal excretion. D- and L-lactic acid are found in many fermented milk products such as yoghurt and cheese, and also in pickled vegetables, and cured meats and fish. The D- and L-lactic acid (generated by bacteria) is a quality indicator of foods, such as egg, milk, fruit juice and wine. Abnormal high concentration of D-lactate in the blood is usually a reflection of bacterial overgrowth in the gastrointestinal tract. AAT Bioquest's Amplite™ Lactate Assay Kits (Cat# 13814 and 13815 for L-lactate assay, and Cat# 13810 and 13811 for D-lactate assay) provide both fluorescence and absorbance-based method for detecting either L-lactate or D-lactate in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, lactate is proportionally related to NADH, which is specifically monitored by a chromogenic NADH sensor. The signal can easily read by an absorbance microplate reader at the absorbance ratio of ~A575nm/A605nm to increase assay sensitivity. With this Colorimetric Amplite™ L-Lactate Assay Kit, we were able to detect as little as 4 µM L-lactate in a 100 µL reaction volume.
Components
Component A: Enzyme Probe                     2 bottles (lyophilized powder)
Component B: Assay Buffer                        1 bottle (10 mL)
Component C: NAD                                    1 vial
Component D: L-Lactate Standard             2.25 mg/vial
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