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- 详细信息
- 文献和实验
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- 保存条件:
详见说明
- 保质期:
详见说明
- 库存:
50株
- 供应商:
上海博尔森生物科技有限公司
- 规格:
10×100ul/50×100ul
| 规格: | 10×100ul | 产品价格: | ¥240.0 |
|---|---|---|---|
| 规格: | 50×100ul | 产品价格: | ¥1020.0 |
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文献和实验Preparing chemically competent cells
Materials Plate of cells to be made competent TSS buffer LB media Ice Glassware & Equipment Falcon tubes 500μl Eppendorf tubes, on ice 200ml conical flask 200μl pipetman or repeating
Transforming chemically competent cells
Method Thaw TSS cells on ice. Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). Note: If you are adding small volumes (~1μl), be careful to mix the culture well. Diluting the plasmid
TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
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