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文献和实验蛋白质和多肽MALDI-MS分析方法 General Method for MALDI-MS Analysis of Proteins and Peptides
on the sample tube wall and/or pipette tip, thereby yielding increased peptide peak number and improved sequence coverage. MALDI-MS matrix solution 20 mg/ml matrix (e.g., HCCA, DHB, or SA)
反向微柱的准备Preparation of Reversed-Phase Microcolumns
tube. The preferred MALDI matrices used for eluting protein from the column are either SA or DHB dissolved in 50% acetonitrile/0.1% TFA (see recipe for protein matrix solution). For eluting peptides from the column, the peptide matrix
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