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文献和实验Matrix deposition is a critical step in tissue imaging by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). It greatly affects the quality of MALDI imaging, especially for the analytes (such as lipids) that may easily
Transgene Integration: Use of Matrix Attachment Regions
Matrix attachment regions (MARs) are operationally defined as DNA elements that bind specifically to the nuclear matrix in vitro. When MARs are positioned at the 5′- and 3′-ends of a transgene higher more predictable expression
when laser desorbed, due to their strong absorption of laser photons. Results of laser desorption experiments indicated that the upper mass limit was ∼5000 Daltons. Matrix-assisted laser desorption/ionization (MALDI) was developed to circumvent the problem
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