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文献和实验Attachment of Nitroxide Spin Labels to Nucleic Acids for EPR
characterization as well as dynamic structural changes of macromolecular systems. To do so, free radicals with good stability must be introduced. Here, the site?directed spin labeling of DNA and RNA based on the Sonogashira cross?coupling reaction is described
Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
线虫蛋白提取--Making Protein Gel Samples from Worms
for other strains to catch up. 6A. If you don't need embryos in your sample, can just add 1 ml gel sample buffer and boil the tube (use a screw cap eppendorf) for 5 minutes, spin the tube 10 min. to pellet debris, and transfer the supernatant to a new tube
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