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文献和实验Ammonium Sulfate Binding Assay for Cells
1. Resuspend cells (vegetative or developed) in phosphate buffer at 1 x 10 8 /ml . 2. Add 50 ul cells to 50 ul 20 nM 3H-cAMP in 5-10 mM DTT (for background binding, add same amount of hot cAMP plus 10 uM cold cAMP) in 1.5 ml eppendorf tube
Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration
and temperature of the solution. Immunoglobulins precipitate at 40–50% ammonium sulfate saturation depending somewhat on the species and subclass (3 ). The desired saturation is brought about either by addition of solid ammonium sulfate or by addition
Antibody Purification: Ammonium Sulfate Fractionation or Gel Filtration
as a convenient first step in antibody purification in that, it allows the concentration of the starting material and the precipitation of the desired protein. The principle of ammonium sulfate precipitation lies in “salting out” proteins from the solution
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