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文献和实验other derivatisation chemistries. Post-hydrolysis extraction of amino acids from PVDF Add 180 µl of fresh extraction solution (60% v/v acetonitrile in 0.01% v/v trifluoroacetic acid) to each autosampler vial containing a PVDF spot. Make sure the PVDF
Cycle Sequencing Protocol Using 33P-Radiolabeled Terminators
tends to produce high background and ghost bands.) 1.Prepare the 4 termination mixes by combining 2.0ul of dGTP-termination-master-mix and 0.5ul of labeled ddNTP for each sequence. 2.For each set of sequencing reactions, label four PCR grade microfuge
反向微柱的准备Preparation of Reversed-Phase Microcolumns
(sinapinic acid) DHB (2,5-dihydroxybenzoic acid) TFA (trifluoroacetic acid) Acetonitrile Dissolve SA or DHB in 50% acetonitrile containing 0.1% TFA.
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