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文献和实验thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure
Immunohistochemistry: Mouse Anti-Human MCP-1
antigen retrieval with 1mM EDTA pH 8.0 at 123?C for 3 minutes in a Pascal pressure chamber (Dako). Wash the slide twice for three minutes each (50mM Tris-HCl pH 8.0, 150mM NaCl in distilled water). 3. Incubate the tissue section with 1% BSA/PBS block
Activation and Expansion of Human Treg Cells
一、实验原理 Dynabeads Human Treg Expander offers a simple method for expansion of Treg cells that does not require antigen- presenting cells or antigen. Just add Dynabeads? Human Treg Expander and recombinant IL-2 (rIL-2) to the Treg culture
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