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文献和实验Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
flasks at approximately 1.3x104 cells/cm2 in Dulbeccos Modified Eagles Medium (DMEM) with 10% Fetal Bovine Serum (FBS). Culture cells for two days in an incubator at 37 °C and 5% CO2 . 2) Make fluorescent g-actin stock solution
concentration of 20 to 200 micrograms/ml Prepare standards containing a range of 20 to 200 micrograms protein (albumin or gamma globulin are recommended) to a standard volume (generally 1 ml or less). See how to prepare and use a protein standard
or gamma globulin are recommended)in 100 µl volume.See how to set up an assay for suggestions as to setting up the standards.5.Add 5 ml dye reagent and incubate 5 min.6.Measure the absorbance at 595 nm.AnalysisPrepare a standard curve of absorbance
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