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文献和实验Isolation of Microtubules (Bovine Brain)
minutes at 4° C for further clarification. Collect 10 ml of the supernatant and determine the protein content of your sample using the Bradford protein assay (Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard
Isolation of Microtubules (Bovine Brain)
content of your sample using the Bradford protein assay ( Appendix G ). Use bovine serum albumin or lysozyme for establishing a standard curve. Decant the remainder of the crude supernatant into a chilled beaker, and add an equal
Creating Transient Cell Membrane Pores Using a Standard Inkjet Printer
flasks at approximately 1.3x104 cells/cm2 in Dulbeccos Modified Eagles Medium (DMEM) with 10% Fetal Bovine Serum (FBS). Culture cells for two days in an incubator at 37 °C and 5% CO2 . 2) Make fluorescent g-actin stock solution
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